干旱胁迫下麻疯树毒蛋白的Western杂交分析

用 10 %～ 4 0 %聚乙二醇 (PEG 6 0 0 0 )处理麻疯树幼苗进行蛋白质诱导 .SDS PAGE显示 ,在根、茎、叶中分别诱导出分子量大约为 17和 30kD、17和 35kD及 15、17、32、5 3、5 6、6 1和 6 5kD的蛋白质多肽链 .用麻疯树毒蛋白(curcin)的抗血清与诱导蛋白进行Western杂交 .结果显示 ,在叶的诱导蛋白中出现分子量为 32和 6 5kD的阳性反应带 ,而在根、茎中无 .这一结果表明干旱胁迫下麻疯树蛋白质分子标记的存在 ,为逆境蛋白的研究奠定了实验基础 .

Western blotting analysis of curcin from Jatropha curcas upon drought stress

Seedlings of Jatropha curcas were treated with 10%-40% polyethylene glycol(PEG 6000) for protein induction. The results show that polypeptides with molecular weights of approximately 17 and 30 kD are induced from roots, 17 and 35 kD from stems, and 15, 17, 32, 53, 56, 61 and 65 kD from leaves on SDS_PAGE gel. Western blottings were performed between antiserum to curcin and induction proteins. Antiserum to curcin recognizes two bands with molecular weights of 32 and 65 kD on all Western blots of inducted proteins from leaves. No band is shown on Western blottings of inducted roots and stems. This result indicates the presence of protein molecular marking under drought stress. This paper lays a foundation for further study on stressed protein.