| 不同来源辣椒疫霉菌的系统发育分析 |
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| 引用本文： | 孙文秀,张修国.不同来源辣椒疫霉菌的系统发育分析[J].河南农业科学,2009(2). |
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| 作者姓名： | 孙文秀 张修国 |
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| 作者单位： | 1. 长江大学,生命科学学院,湖北,荆州,434025
2. 山东农业大学,植物保护学院,山东,泰安,271018 |
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| 基金项目： | 山东省优秀中青年科学家研究奖励基金 |
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| 摘 要： | 运用PCR直接测序法,对9个辣椒疫霉菌株和1个外类群大豆疫霉菌株的nrDNA的ITS区(包括ITS1、5.8SrDNA和ITS2)进行序列测定。结果表明,不同来源的辣椒疫霉菌的ITS序列总长度为750~753bp。采用PAUP软件进行系统发育分析表明:来自辣椒寄主和土壤的辣椒疫霉菌各自聚为一组,与其地理来源没有明显的相关性。
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| 关 键 词： | 辣椒疫霉菌 ITS序列分析 系统发育 |
Phylogenetic Analysis of Phytophthora capsici from Different Areas |
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| SUN Wen-xiu,ZHANG Xiu-guo.Phylogenetic Analysis of Phytophthora capsici from Different Areas[J].Journal of Henan Agricultural Sciences,2009(2). |
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| Authors: | SUN Wen-xiu ZHANG Xiu-guo |
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| Institution: | 1.College of Life Science;Yangtze University;Jingzhou 434025;China2.College of Plant Protection of Shandong Agricultural University;Taian 271018;China |
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| Abstract: | The ITS region(including ITS1,5.8S rDNA and ITS2) of nuclear ribosomal DNA from 9 isolates of Phytophthora capsici and one outgroup isolate of P.sojae were sequenced.The result showed that the sizes of the ITS region of P.capsici strains from different sources were from 750 to 753 bp.Based on the phylogenetic analysis of nucleotide sequences with PAUP,the strains of P.capsici from pepper hosts and soil were gathered respectively.The division of isolates of P.capsici was not related to their geographical ori... |
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| Keywords: | Phytophthora capsici ITS sequence analysis Phylogeny |
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