抗大豆胞囊线虫SCN3-11位点的KASP标记开发和利用

大豆胞囊线虫(Heterodera glycines Ichinohe)是严重危害世界范围大豆生产的害虫, 采用合理轮作和种植抗病品种可有效控制损失。为了开展分子标记辅助选择以加速抗病品种培育, 本研究针对前期发现的与大豆胞囊线虫3号小种(SCN3)抗性显著关联的非同义变异SNP位点Map-5149, 开发高通量、低成本的新型分子标记—竞争性等位基因特异PCR标记(kompetitive allele specific PCR, KASP), GmSNAP11-5149。利用GmSNAP11-5149鉴定了来自8个国家的202份代表性大豆抗感资源, 发现141份材料携带抗病基因型GmSNAP11-5149-AA, 平均雌虫指数为6.2%, 极显著低于58份携带感病基因型GmSNAP11-5149-GG材料的雌虫指数(61.1%), 方差分析表明, GmSNAP11-5149与胞囊线虫的抗性显著相关(F=44.18, P<0.0001), 对抗病材料的选择效率达到92%, GmSNAP11-5149可作为一个实用的分子标记应用于辅助抗大豆胞囊线虫品种选育和抗病种质资源鉴定。

Development and Utilization of KASP Marker for SCN3-11 Locus Resistant to Soybean Cyst Nematode

Soybean cyst nematode (Heterodera glycines Ichinohe) is one of the most economically destructive pathogens worldwide. The cultivation of resistant soybean cultivars combined with crop rotation was the most effective management strategy to reduce yield losses due to SCN. To accelerate the breeding of cultivars resistant to SCN under molecular marker assisted selection, we developed an economical, time-saving and high-throughput KASP marker GmSNAP11-5149 by using a reported non-synonymous polymorphism (Map-5149) on the minor SCN3-11 resistant to SCN3. Two hundred and two soybean accessions from eight countries with known SCN3 were genotyped for GmSNAP11-5149. The average female index of 141 accessions carrying GmSNAP11-5149-AA (6.2%) was significantly lower than that of 58 accessions carrying GmSNAP11-5149-GG (61.1%) at the P < 0.0001 probability level. The selection efficiency of GmSNAP11-5149 for resistant accessions was 92%. It is suggested that GmSNAP11-5149 could be used to effectively distinguish resistant genotypes from susceptible ones in the tested soybean accessions and subsequently could be used in molecular marker-assisted breeding and identification of resistant germplasm.