Living cells as test tubes |
| |
Authors: | Xie X Sunney Yu Ji Yang Wei Yuan |
| |
Institution: | Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA. xie@chemistry.harvard.edu |
| |
Abstract: | The combination of specific probes and advanced optical microscopy now allows quantitative probing of biochemical reactions in living cells. On selected systems, one can detect and track a particular protein with single-molecule sensitivity, nanometer spatial precision, and millisecond time resolution. Metabolites, usually difficult to detect, can be imaged and monitored in living cells with coherent anti-Stokes Raman scattering microscopy. Here, we describe the application of these techniques in studying gene expression, active transport, and lipid metabolism. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|