Bruchpilot promotes active zone assembly, Ca2+ channel clustering, and vesicle release |
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Authors: | Kittel Robert J Wichmann Carolin Rasse Tobias M Fouquet Wernher Schmidt Manuela Schmid Andreas Wagh Dhananjay A Pawlu Christian Kellner Robert R Willig Katrin I Hell Stefan W Buchner Erich Heckmann Manfred Sigrist Stephan J |
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Affiliation: | European Neuroscience Institute G?ttingen, Grisebachstrasse 5, 37077 G?ttingen, Germany. |
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Abstract: | The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release and patterned synaptic plasticity. |
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