| 奶牛朊病毒基因克隆与序列分析 |
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| 引用本文： | 杨建民,赵德明,郝永新,李宁,于书敏,秦秀慧. 奶牛朊病毒基因克隆与序列分析[J]. 畜牧兽医学报, 2004, 35(6): 685-688 |
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| 作者姓名： | 杨建民 赵德明 郝永新 李宁 于书敏 秦秀慧 |
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| 作者单位： | 1. 中国农业大学动物医学院国家动物海绵状脑病实验室,北京,100094
2. 中国农业大学农业生物技术国家重点实验室,北京,100094 |
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| 基金项目： | 国家自然科学基金项目(30371062，30400325)，教育部博士点基金(20020019006) |
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| 摘 要： | 根据已报道正常牛朊蛋白(PrP^c)基因(PRNP)序列设计引物，采用PCR法扩增了6头荷斯坦奶牛的PRNP基因，将其克隆到T-Vector。序列测定及分析表明所克隆的奶牛PRNP基因片段为795bp，该基因内无内含子，包含了牛PRNP完整编码区序列，编码264个氨基酸的前体蛋白，推测其分子量约34ku。其中2头共同含有未曾报道的牛PRNP多态性位点M120I，无义突变G234A，但未引起酶切位点变异，未发现插入或缺失变异；与已报道牛PRNP序列(GenBank收录号为DI0613)相比，两者核苷酸序列同源性为99％，其编码的氨基酸同源性为99％。
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| 关 键 词： | 基因克隆 朊病毒 无义突变 正常 克隆 PCR法 前体蛋白 荷斯坦奶牛 序列分析 变异 |
| 文章编号： | 0366-6964(2004)06-0685-04 |
Cloning and Sequencing of Cattle PRNP |
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| YANG Jian-min,ZHAO De-ming,HAO Yong-xin,LI Ning,YU Shu-min,QIN Xiu-hui. Cloning and Sequencing of Cattle PRNP[J]. Chinese Journal of Animal and Veterinary Sciences, 2004, 35(6): 685-688 |
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| Authors: | YANG Jian-min ZHAO De-ming HAO Yong-xin LI Ning YU Shu-min QIN Xiu-hui |
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| Affiliation: | YANG Jian-min~1,ZHAO De-ming~1,HAO Yong-xin~1,LI Ning~2,YU Shu-min~1,QIN Xiu-hui~1 |
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| Abstract: | The total DNA was isolated from lymphocyte of peripheral blood of cattle. The PRNP gene was amplified by PCR method. The cloned PRNP gene was 795 nucleotides long and contains the entire PRNP coding sequence which has no intron and has 99% homology with the published gene sequence. And the deduced protein contain 264 amino acids, which had a calculated molecular weight (MW) of 34.0 ku. It is the first time the new alleles,Q264Q and G360A were found in the coding region of PRNP. |
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| Keywords: | cattle PRNP prion |
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