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| 棉花黄萎病菌gDNA提取方法和不同单孢的RAPD分析 | |
| 引用本文： | 刘学堂,郭金城,张元恩,宋晓轩,孙君灵,李为.棉花黄萎病菌gDNA提取方法和不同单孢的RAPD分析[J].河南农业大学学报,1999,33(3):274-278. |
| 作者姓名： | 刘学堂 郭金城 张元恩 宋晓轩 孙君灵 李为 |
| 作者单位： | 1. 中国农业科学院棉花研究所农业部棉花遗传改良重点实验室,河南,安阳,455112 2. 中国农业大学植物科技学院植病系,北京,100094 3. 河南农业大学,河南,郑州,450002 |
| 摘 要： | 选用棉花黄萎病菌代表性的菌系Ｖａ（Ｖｅｔｉｃｌｌｉｕｍａｌｂｏａｔｒｕｍ的一个菌株）和Ｖｅｔｉｃｉｌｌｉｕｍｄａｈｌｉａｅ的落中型菌系ＶＤ－８，Ｔ９及非落 叶型菌系Ｊｙ，以Ｋｅ作为实验对象，研究了ＣＴＡＢ和氯化苄２种提取方法的优劣。 |
| 关 键 词： | 棉花黄萎病菌 DNA提取 单孢 RAPD分析 |
Studies on methods of gDNA extraction and RAPD analysis of cotton Verticillium wilt pathogen |
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| LIU Xue tang ,GUO Jin cheng ,ZHANG Yuan en ,et al.Studies on methods of gDNA extraction and RAPD analysis of cotton Verticillium wilt pathogen[J].Journal of Henan Agricultural University,1999,33(3):274-278. | |
| Authors: | LIU Xue tang GUO Jin cheng ZHANG Yuan en |
| Institution: | LIU Xue tang 1,GUO Jin cheng 1,ZHANG Yuan en 2,et al |
| Abstract: | The study on the methods of DNA extraction showed that CTAB method is better than PhCH 2Cl method. DNA extracted by CTAB method has longer fragment, higher purity, better stability, and less pollution. So it is suitable for the conventional DNA extraction of cotton Verticillium wilt pathogen. The primers screening of RAPD analysis has been performed with 40 primers (OPY, OPB) and 16 primers amplified polymorphic DNA patterrn. RAPD analysis of different single spores also proves that the genetic variation may occur among different single spore from one species. |
| Keywords: | cotton Verticillium wilt pathogen DNA extraction single spore RAPD analysis |
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