苜蓿素对AA肉仔鸡免疫指标的影响

为研究不同剂量的苜蓿素对AA肉仔鸡免疫指标的影响,选取1日龄AA肉仔鸡120只,随机分为4组,对照组饲喂基础日粮,试验Ⅰ~Ⅲ组基础日粮中分别添加150、300、450 mg/kg苜蓿素。分别于28、35和42 d从每组取10只鸡进行采血,测定血清球蛋白含量;取法氏囊和脾脏,称重并计算免疫器官指数;采用MTT比色法测定T、B淋巴细胞增殖能力。结果表明,基础日粮中添加300 mg/kg苜蓿素饲喂28 d,能够显著提高AA肉仔鸡法氏囊指数（P〈0.05）;添加450 mg/kg苜蓿素饲喂28 d,能够显著提高AA肉仔鸡血清球蛋白含量和免疫器官指数（P〈0.05）。添加300 mg/kg苜蓿素饲喂35 d,能够显著提高AA肉仔鸡血清球蛋白含量、免疫器官指数（P〈0.05）,显著促进B淋巴细胞增殖（P〈0.05）;添加450 mg/kg苜蓿素饲喂35 d,能够显著提高血清球蛋白含量、脾脏指数（P〈0.05）,显著促进T淋巴细胞增殖（P〈0.05）,极显著促进B淋巴细胞增殖（P〈0.01）。添加300和450 mg/kg苜蓿素饲喂42 d,能够极显著提高AA肉仔鸡法氏囊指数（P〈0.01）;添加450 mg/kg苜蓿素饲喂42 d,能够显著促进B淋巴细胞增殖（P〈0.05）。结果提示,日粮中添加苜蓿素能够提高多项AA肉仔鸡免疫指标,以添加450 mg/kg效果最优。

Effect of Tricin on Immunological Indicators of AA Broiler

In order to investigate the effect of tricin on immunological indicators of AA broiler, a total of 120 AA broilers were selected and randomly divided into four groups. The control group was fed with basal diet. The basal diet of experimental groupⅠ, Ⅱ and Ⅲ were supplemented with 150, 300 and 450 mg/kg tricin, respectively. Ten broilers were chosen from each group in28 th, 35 thand 42thd to determine their serum globulin content. Meanwhile, the bursa of Fabricius and spleen of the selected broilers were collected and weighed to calculate their immune organs index, and the proliferation ability of T and B lymphocyte were determined by using MTT colorimetry. The results showed that the index of bursa of Fabricius of AA broilers were significantly increased when they were fed with the basal diet supplemented with 300 mg/kg of tricin for 28 d（P〈0.05）. The serum globulin level and immune organs index of AA broilers were significantly increased when they were fed with the basal diet supplemented with 450 mg/kg of tricin for 28 d（P 0.05）. The serum globulin level, immune organs index and the proliferation ability of B lymphocyte of AA broilers were significantly increased when they were fed with the basal diet supplemented with 300 mg/kg of tricin for 35 d（P〈0.05）. The serum globulin level, spleen index and the proliferation ability of T and B lymphocyte of AA broilers were significantly increased when they were fed with the basal diet supplemented with 450 mg/kg of tricin for 35 d（P〈0.05 or P〈0.01）. The index of bursa of Fabricius of AA broilers were significantly increased when they were fed with the basal diet supplemented with 300 and 450 mg/kg of tricin for 42 d（P〈0.01）, respectively. The proliferation ability of B lymphocyte of AA broilers were significantly increased when they were fed with the basal diet supplemented with 450 mg/kg of tricin for 42 d（P〈0.05）. It was indicated that a series of immunological indicators of AA broiler were increased when they were fed with the basal diet supplemented with tricin, and the optimum adding quantity was 450 mg/kg.