CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry |
| |
| Authors: | Vig M Peinelt C Beck A Koomoa D L Rabah D Koblan-Huberson M Kraft S Turner H Fleig A Penner R Kinet J-P |
| |
| Affiliation: | Department of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA. mvig@bidmc.harvard.edu |
| |
| Abstract: | Store-operated Ca2+ entry is mediated by Ca2+ release-activated Ca2+ (CRAC) channels following Ca2+ release from intracellular stores. We performed a genome-wide RNA interference (RNAi) screen in Drosophila cells to identify proteins that inhibit store-operated Ca2+ influx. A secondary patch-clamp screen identified CRACM1 and CRACM2 (CRAC modulators 1 and 2) as modulators of Drosophila CRAC currents. We characterized the human ortholog of CRACM1, a plasma membrane-resident protein encoded by gene FLJ14466. Although overexpression of CRACM1 did not affect CRAC currents, RNAi-mediated knockdown disrupted its activation. CRACM1 could be the CRAC channel itself, a subunit of it, or a component of the CRAC signaling machinery. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
