一氧化氮供体SNP对花生萌发的影响 |
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引用本文: | 孙新,胡彦江. 一氧化氮供体SNP对花生萌发的影响[J]. 安徽农业科学, 2010, 38(12): 6100-6102. DOI: 10.3969/j.issn.0517-6611.2010.12.011 |
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作者姓名: | 孙新 胡彦江 |
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作者单位: | 青岛农业大学生命科学学院,山东青岛,266109;青岛农业大学生命科学学院,山东青岛,266109 |
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基金项目: | 青岛农业大学高层次人才科研启动基金 |
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摘 要: | [目的]阐明一氧化氮供体SNP(sodium nitroprusside)对花生种子萌发的作用与活性氧以及淀粉酶同工酶、酯酶同工酶活性的关系。[方法]采用组织化学原位染色和电泳的方法。[结果]经100μmol/L SNP处理的花生的萌发过程受到明显的抑制,组织内H2O2、O2-.的含量及α-淀粉酶的活性均有明显降低,而β-淀粉酶、酯酶同工酶的活性无明显变化。[结论]SNP对花生种子萌发的抑制作用与组织中活性氧浓度的降低有关。
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关 键 词: | 花生 一氧化氮 同工酶 |
Nitric Oxide Donor SNP on the Germination of Peanut |
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Affiliation: | SUN Xin et al(Life Science College,Qingdao Agricultural Univerty,Qingdao,Shandong 266109) |
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Abstract: | [Objective]The aim was to illustrate the relationships between reactive oxygen species contents and amylase and esterase isozymes activities during peanut germination under the treatment of SNP,which was nitric oxide donor.[Method] Histochemical method and electrophoresis were applied,respectively.[Result] 100 μmol/L SNP played a significant role in inhibiting the germination of peanut,as compared to control.The H2O2,O-2· contents and the activities of α-amylase all decreased in the presence of SNP,while the activities of esterase and β-amylase were remained the same.[Conclusion]The inhibition on peanut germination in the present of SNP linked to the decrease of reactive oxygens contents. |
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Keywords: | Peanut Nitric oxide Isozyme |
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