Biochemical characterization of lipopolysaccharides extracted from a hydrophobic strain of Pasteurella multocida |
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Authors: | R. S. Conrad C. Galanos F. R. Champlin |
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Affiliation: | (1) Department of Biochemistry and Microbiology, Oklahoma State University College of Osteopathic Medicine, 1111 West 17th Street, 74107-1898 Tulsa, OK, USA;(2) Max-Planck-Institut für Immunbiologie, 79108 Freiburg-Zähringer, Germany;(3) Department of Biological Sciences, Mississippi State University, PO Drawer GY, 39762 Mississippi State, MS, USA |
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Abstract: | Lipopolysaccharides were extracted from freeze-dried cells of Pasteurella multocida strain P-1581 (serotype 8) by the phenol-chloroform-petroleum ether method and biochemically analysed using standard procedures. The primary neutral sugars were glucose, galactose and heptose. No deoxy sugars were detected. Amino sugars included galactosamine, glucosamine and glucosamine-6-phosphate. 3-Deoxy-d-manno-2-octulosonic acid was present at a relatively low concentration. The analyses included identification and quantification of phosphate and alanine. The primary fatty acids and their approximate relative ratios were 3-hydroxytetradecanoate and tetradecanoate 2:1. Tetradecanoic acid was bound almost exclusively by ester linkages. 3-Hydroxytetradecanoic acid was bound primarily by amide linkages, although significant numbers of ester-bound residues were noted. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analyses indicated that the lipopolysaccharides were of low molecular weight.Abbreviations KDO 3-deoxy-d-manno-2-octulosonic acid - LPS lipopolysaccharide(s) - SDS-PAGE sodium dodecyl sulphate-polyacrylamide gel electrophoresis |
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Keywords: | cell membrane electrophoresis gas-liquid chromatography lipopolysaccharides PAGE Pasteurella multocida |
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