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细胞色素P450s的纯化与鉴定
引用本文:郑明奇,张文吉,邱星辉. 细胞色素P450s的纯化与鉴定[J]. 农药学学报, 2001, 3(3): 11-17
作者姓名:郑明奇  张文吉  邱星辉
作者单位:1.中国农业大学应用化学学院, 北京 100094
摘    要:综述了细胞色素 P450s纯化和鉴定技术的研究进展。纯化技术包括色谱、电泳和基因克隆技术 ;鉴定方法有光谱测定、酶活测定、电泳分析和蛋白质印迹测定。

关 键 词:细胞色素P450s   纯化   鉴定

Purification and Identification of Cytochrome P450s
Zheng Ming-qi,Zhang Wen-ji and Qiu Xing-hui. Purification and Identification of Cytochrome P450s[J]. Chinese Journal of Pesticide Science, 2001, 3(3): 11-17
Authors:Zheng Ming-qi  Zhang Wen-ji  Qiu Xing-hui
Affiliation:1.College of Applied Chemistry2.China Agricultural University
Abstract:A brief review on purification and identification of specific cytochrome P450s is given. Purification technologies include chromatography,electrophoresis and gene cloning. Identification methods include spectral determination, enzymatic analysis, electrophoretic analysis, and western blotting.
Keywords:Cytochrome P450s  Purification  Identification
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