| 鸭新城疫病毒山东株的分离鉴定 |
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| 引用本文： | 张太翔,王炳军,王裕,田国华,田国宁,张金玲,韩光来,韩亮,张丽萍. 鸭新城疫病毒山东株的分离鉴定[J]. 中国畜牧兽医, 2009, 36(7): 148-151 |
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| 作者姓名： | 张太翔 王炳军 王裕 田国华 田国宁 张金玲 韩光来 韩亮 张丽萍 |
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| 作者单位： | (1.山东潍坊出入境检验检疫局， 潍坊 261041； 2.潍坊职业学院， 潍坊 261041) |
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| 基金项目： | 山东出人境检验检疫局科研课题 |
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| 摘 要： | 笔者从病鸭体内分离到一株病毒，通过试验确定为新城疫病毒。参照世界动物卫生组织（OIE）规定的新城疫毒力判定的标准和方法，测定该分离株的1日龄雏鸡脑内致病指数（ICPI）和6周龄鸡静脉致病指数（IVPI）分别为1.79和2.45。采用已经发表的鸡新城疫病毒序列设计引物，应用RT-PCR技术扩增鸭源新城疫病毒的F基因，并将其克隆至载体上，然后进行了核苷酸序列、氨基酸序列测定，结果表明该分离株为新城疫强毒株。
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| 关 键 词： | 鸭源新城疫 分离鉴定 F基因 RT-PCR 序列分析 |
Isolation and Identification of Newcastle Disease Virus in Duck in Shandong Province |
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| ZHANG Tai-xiang,WANG Bing-jun,WANG Yu,TIAN Guo-hua,TIAN Guo-ning,ZHANG Jin-ling,HAN Guang-lai,HAN Liang,ZHANG Li-ping. Isolation and Identification of Newcastle Disease Virus in Duck in Shandong Province[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(7): 148-151 |
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| Authors: | ZHANG Tai-xiang WANG Bing-jun WANG Yu TIAN Guo-hua TIAN Guo-ning ZHANG Jin-ling HAN Guang-lai HAN Liang ZHANG Li-ping |
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| Affiliation: | (1.Weifang Entry-Exit Inspection and Quarantine Bureau of People’s Republic of China, Weifang 261041, China; 2.Weifang Vocational Institute, Weifang 261041, China) |
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| Abstract: | The virus isolated from duck was identified as Newcastle disease virus by the HA/HI.The intracerebral pathogenicity index in 1-day-old chickens,the intravenous pathogenicity 6-week-old chickens were 1.79 and 2.45 according to the standard and methods of Newcastle disease pathogenicity judgment refer to OIE.We amplified its fusion protein gene by RT-PCR using the primer which got from the reported sequence of NDV in GenBank,cloned into the carrier and sequenced.The results showed that the isolated virus was ... |
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| Keywords: | RT-PCR |
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