稻瘟病菌株的DNA指纹及其与小种致病性相互关系的研究

稻瘟病菌生理小种的鉴别对培育抗稻瘟病水稻品种具有重要意义。传统方法利用一套鉴别品种区分小种既复杂又易受各种因素的影响。我们在日本稳定菌株北1的基因组文库中筛选出了一套含重复顺序的克隆，2 个克隆被证实具有高度的多态性。用其中的POR6对17个稻瘟病菌株Southern分析，获得了可分辨和重复的基因组特异的指纹图谱。

DNA Fingerprinting and It''''s Relationship with the Pathogenicity in Rice Blast Fungus

Identification of rice blast fungus is important to breeding blast-resistant rice. The traditional method for assign ment of the blast pathotypes is complicated and its results are vulnerable to the ef-fects of various factors. In order to develop a precise identification method based on DNA fingerprints, a set of clones containing repetitive sequences has been selected from a genomic library which was con-structed from & Japanese pathotype Hoku 1. Two of them were verified to have highly polymorphisms among 17 rice blast isolates. out Southern analysis with POR6 as probe,distinctive and reproducible genome-specific DNA-fingerprints were observed. In this paper,the relationship between pathotypes and DNA-fingerprints of 8 blast isolates is shown. The results indicate that the percentage of similar hybridizing bands (Sxy) in POR6-DNA fingerprints of any two isolates are positively related to the number of rice cultivars which can be infected by both of them.