| 高粱非编码区SSR引物设计以及e-PCR的验证 |
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| 引用本文： | 陆景标,李杰勤,卢杰,詹秋文.高粱非编码区SSR引物设计以及e-PCR的验证[J].种子,2010,29(9). |
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| 作者姓名： | 陆景标 李杰勤 卢杰 詹秋文 |
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| 作者单位： | 安徽科技学院;安徽农业大学; |
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| 基金项目： | 国家自然科学基金，科技部农业科技成果转化资金项目，安徽省"115"产业创新团队资助项目，国家科技支撑计划项目，安徽省高校学科拔尖人才基金，安徽科技学院稳定人才专项项目 |
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| 摘 要： | 本研究通过在NCBI公共数据库的高粱全基因序列中进行简单重复序列SSR搜索,结果显示共有87950个SSR位点,共有1134种重复,其中二元碱基和三元碱基重复所占比例最大,分别占43.36%,32.38%。根据SSR搜索结果设计了高粱SSR引物,通过两次e-PCR验证和筛选引物,随机合成了50对引物。以先锋和乐食两个高丹草品种为材料进行引物的筛选和评价。结果表明,50对引物中有46对引物出带,但只有8对引物具有多态性。这说明在高粱非编码区设计的引物多态性并不高于EST-SSR引物,但对高粱的SSR引物的开发可以起到补充和促进作用。
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| 关 键 词： | 高粱 非编码区 SSR e-PCR 多态性 |
Design of SSR Primers and Verification of E-PCR in Non-coding Regions of Sorghum Genome |
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| LU Jing-biao,LI Jie-qin,LU Jie,ZHAN Qiu-wen.Design of SSR Primers and Verification of E-PCR in Non-coding Regions of Sorghum Genome[J].Seed,2010,29(9). |
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| Authors: | LU Jing-biao LI Jie-qin LU Jie ZHAN Qiu-wen |
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| Institution: | LU Jing-biao1,2,LI Jie-qin1,LU Jie1,ZHAN Qiu-wen1(1.Anhui Science and Technology University,Fengyang Anhui 233100,China,2.Anhui Agricultural University,Hefei 230036,China) |
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| Abstract: | The complete genome sequence of sorghum which downloaded from the NCBI public databases was used for a simple sequence repeated SSR search.The result showed that there were 87950 SSR loci and 1134 kinds of repetition about the results in all.The proportion of dinucleotide and trinucleotide repeats was placed on the first place,accounting for 43.36% and 32.38% of the all.SSR primers of sorghum were designed basing on the search results of SSR,according to use the e-PCR to validate and screen primers two time... |
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| Keywords: | sorghum non-coding regions SSR e-PCR polymorphism |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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