子宫内膜炎患牛子宫黏液内念珠菌的分离及体外溶血活性鉴定

本研究通过API20CAUX系统对从子宫内膜炎患牛子宫内黏液中分离到的183株念珠菌进行鉴定。结果显示克鲁斯念珠菌最多,为33.9%,其次是皱褶念珠菌为17.5%,乳酒念珠菌为13.1%,白色念珠菌为11.5%及热带念珠菌为8.7%。分离中不常见的有假丝酵母为5.5%,近平滑念珠菌为4.4%,吉利蒙念珠菌为3.3%,著名念珠菌为1.1%和光滑念珠菌为1.1%。此外,还对所分离到的念珠菌进行体外溶血活性检测,结果显示克鲁斯念珠菌、乳酒念珠菌、白色念珠菌、热带念珠菌、涎沫假丝念珠菌和光滑念珠菌在接种后48 h后显示α和β溶血;皱褶念珠菌、吉利蒙念珠菌、著名念珠菌只显示α溶血;在孵育72 h后,近平滑念珠菌不显示任何溶血活性。涎沫假丝酵母、白色念珠菌和乳酒念珠菌的β溶血活性比其他菌株高。本研究为临床牛真菌性子宫内膜炎的防治及发病机理的研究奠定了基础。

Identification of Candida Species and the Hemolytic Activity Isolated from Endometritis Cattle

Candida stain is a conditional pathomycete that could be isolated from the bovine with the disease of endometritis. The 183 Candida strains were isolated from the uterus mucus of bovine with disease endometritis were used to identified at the level of generic by API20CAUX. The most commonly could be isolated were Candida krusei which accounted for 33.9%, followed by Candida rugosa were 17.5%, and the Candida kefyr, Candida albicans, Candida tropicalis were 13.1%, 11.5%, 8.7%. The seldom could be isolated were Candida zeylanoides, Candida parapsilosis, Candida guilliermondi, Candida fanata, Candida glabrata were 5.5%, 4.4%, 3.3%, 1.1%, 1.1%. In addition, we had also tested the active of hemolyzation in vitro of the Candida stain. Candida krusei, Candida kefyr, Candida albicans, Candida tropicalis, Candida zeylanoides and Candida glabrata display α and β haemolysis post inoculation 48 h. Candida rugosa, Candida guilliermondi, Candida fanata only display α haemolysis, Candida parapsilosis didn’t display any haemolysis after incubation 72 h. Candida albicans and Candida kefyr had a higher haemolysis than others. This study lays the foundation for future study of clinical prevention and treatment of fungal endometritis and pathogenesis.