牛磺酸对STZ诱导大鼠胰岛细胞外液中SOD、GSH-PX、MDA及T-AOC含量的影响

试验旨在研究牛磺酸(Tau)对链脲佐菌素(STZ)处理后的胰岛细胞保护作用,探讨牛磺酸防治糖尿病的作用机制,为应用牛磺酸防治糖尿病提供理论依据。将体重为250~300 g大鼠麻醉后,胆总管原位灌注胶原酶V,38 ℃水浴消化胰腺组织。Histopaque 1077密度梯度离心纯化胰岛细胞,DTZ检测纯度,葡萄糖刺激检测胰岛细胞活性;再将分离纯化后的胰岛细胞分成6组:正常对照组(对照组)、牛磺酸对照组、链脲佐菌素组(STZ组) 、牛磺酸高剂量组(20 mmol/L Tau组)、牛磺酸中剂量组(10 mmol/L Tau组)、牛磺酸低剂量组(5 mmol/L Tau组);培养24 h后,测定细胞外液中超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、总抗氧化能力(T-AOC)的含量。结果表明,3个水平的Tau组和Tau对照组胰岛细胞外液中的SOD、GSH-Px、T-AOC的含量较STZ组有明显的提高。同时3个水平的Tau组和Tau对照组胰岛细胞外液中的MDA较STZ组有明显的降低。因此,牛磺酸能够提高STZ诱导的胰岛细胞抗氧化能力,从而对胰岛细胞具有一定的保护和修复作用。

The Impact of Tautrine on SOD,GSH-PX,MAD and T-AOC in Extracellular Fluid of STZ-induced Islet Cell

To approach the protective effects of taurine on islet cells treated with streptozotocin,then provide a theoretical basis for the treatment of diabetes.250 to 300 g rats were in situ perfusion with collagenase V in bile common duct after anesthesia. Pancreatic tissues were digested using aqueous bath under 38 centigrade. Islet cells were purified by Histopaque 1077 density gradient centrifugation,and the purity was detected by DTZ,the cytoactive was detected by glucose stimulation. Purified islet cells were randomly divided into 6 groups,normal control group (control group),taurine control group,streptozotocin group(STZ group),high-dose taurine group (20 mmol/L Tau group),medium-dose taurine group (10 mmol/L Tau group) and low-doses taurine group (5 mmol/L Tau group). The cells were cultured for 24 h,then the level of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),malondialdehyde (MDA),total antioxidant capacity (T-AOC),were measured.The results showed that the three doses of taurin groups and taurine control group could significantly increase the levels of SOD,GSH-Px,T-AOC in extracellular fluid compared with STZ group,and could significantly decrease the level of MDA compared with STZ group.Therefore,taurine could protect and repair islet cells by way of increasing the oxidation resistant ability of islet cells induced with STZ.