响应面法优化多粘菌素B的发酵条件

采用响应面法对多粘类芽孢杆菌(Paenibacillus polymyxa)产多粘菌素B的发酵条件进行了优化。在单因素实验的基础上确定了对产量影响较大的三个因素为初始pH值、发酵温度和发酵时间。采用Box-Behnken实验设计进行响应面分析优化，确定了最佳发酵条件为：初始pH值6.72，温度30.64℃，发酵时间37.72h 。在此条件下进行发酵验证，实验值和模型预测值之间比较接近，有较高的吻合度，相对误差较小。多粘菌素B的产量可达到915.77mg/L，比优化前提高了33.7%。

Optimization of Fermentation Conditons for Polymyxin B Production by Response Surface Method

The fermentation conditions of polymyxin B production by Paenibacillus polymyxa was optimized using response surface method. Through the single factor experiment, three factors were confirmed to have significant effect on polymyxin B production, that is, the initial pH, fermentation temperature and fermentation time. Using Box-Behnken experimental design for response surface optimization, the optimum fermentation conditions were： initial pH value of 6.72, temperature 30.64℃ , fermentation time 37.72 h. Under these conditions, the fermentation validation showed that the experimental value was comparable to model prediction, having high inosculating degree and the error was relatively small. The yield of Polymyxin B could reach 915.77 mg / L, which is 33.7% higher than before optimization.