新疆蟠桃中发现油桃茎痘相关病毒和亚洲李属病毒

采用高通量测序(High-throughput sequencing,HTS)技术检测新疆蟠桃感染病毒的情况。采集具有穿孔、脉间褪绿等症状的蟠桃嫩叶,提取总RNA,用于高通量测序,共获得13.36 Gb的数据,包含44563187对reads,其中比对到油桃茎痘相关病毒(nectarine stem pitting-associated virus,NSPa V,KT273409)和亚洲李属病毒(asian prunus virus,APV2,KT893294)基因组的分别有9943对和40036对,经拼接各得到长4978和9393 nt的contig,基因组覆盖率均接近100%(5′端分别差10个和7个碱基),核酸一致度分别为95.0%和92.9%,将此分离物分别命名为NSPa V-Tao和APV2-Tao4。用RT-PCR方法对23个蟠桃树样品进行了检测,结果NSPa V和APV2检出率分别为43.5%和69.6%。通过比较已知NSPa V病毒的核苷酸序列,发现NSPa V-Tao可能是其他分离物重组的结果。

Nectarine Stem Pitting-associated Virus and Asian Prunus Virus Found in Prunus persica of Xinjiang

In this study,the High-throughput sequencing technology(HTS)was used to detect the virus infection of Prunus persica var.compressa.The peach leaves showing shot hole and chlorosis between leaf veins were collected to extract total RNA for HTS.A total of 13.36 Gb sequence data,including 44563187 paired reads,were obtained.The sequence alignment analysis showed that there were 9943 and 40036 paired reads belong to the genome of nectarine stem pitting-associated virus(NSPa V)(KT273409)and asian prunus virus(APV2)(KT893294).The contigs of 4978 nt and 9393 nt with nearly completed coverage of viruses genome(10 and 7 bases missing at 5′end,respectively)were assembled and the sequence identity with the genome sequence of NSPa V(KT273409)and APV2(KT893294)were 95.0%and 92.9%respectively.We named them as isolates NSPa V-Tao and APV2-Tao4.To confirm the result of HTS,23 samples of P.persica var.compressa were detected by the RT-PCR method for the infection of NSPa V and APV2.The results showed that the infection rate of NSPa V and APV2 was 43.5%and 69.6%,respectively.Moreover,NSPa V-Tao may be a recombination of other NSPa V isolates based on the nucleotide sequence comparison.