樱桃种质SCoT分子标记与叶片表型性状关联分析

利用本研究中建立的樱桃SCoT标记分析体系,进行适宜引物筛选,进而对12个樱桃品种进行SCoT分子标记、叶片表型性状遗传多样性及两者关联分析.结果 显示:从102条SCoT标记引物中筛选出46条适宜樱桃遗传分析的标记引物,筛选率达45.1％;参试的18条SCoT引物共获得等位位点数77个,其中多态性位点65个,多态性比...

Association Analysis of SCoT Markers and Leaf Phenotypic Traits in Cherry Germplasm

Based on the established SCoT analysis system,the suitable primers were screened,and then SCoT molecular markers,genetic diversity of leaf traits and their correlation were analyzed in 12 cherry varieties.The results showed that 46 marker primers being suitable for cherry genetic analysis were selected from 102 SCoT marker primers,with a screening rate of 45.1%.A total of 77 allele points were obtained from 18 SCoT primers,including 65 polymorphic loci,with a polymorphism rate of 84.4%.The variation coefficient of leaf traits of 12 varieties was 11.49%-36.93%,which was basically consistent with the results of SCoT marker clustering analysis.Pearson’s correlation analysis showed that a total of 54 marker sites were significantly or extremely significantly correlated with 12 leaf traits.Through the multiple linear stepwise regression analysis,eight specific marker sites related to leaf traits were obtained,which explained the change of 47.8%-100.0%of leaf traits.Sequence analysis of eight association marker sites showed that five markers could find homologous sequences in cherry genome;the similarity was more than 75%;and their chromosome positions could be identified.Among them,four markers could find homologous sequences with functional annotation in NCBI,and three markers could not find homologous sequences.