| 马铃薯CHI家族基因鉴定及其对外源水杨酸和茉莉酸的响应分析 |
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| 引用本文： | 荐红举,万梦圆,付毅,丁艺,琚熙三,尚丽娜,李辉,王季春,胡柏耿,吕典秋.马铃薯CHI家族基因鉴定及其对外源水杨酸和茉莉酸的响应分析[J].园艺学报,2021(1):83-95. |
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| 作者姓名： | 荐红举 万梦圆 付毅 丁艺 琚熙三 尚丽娜 李辉 王季春 胡柏耿 吕典秋 |
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| 作者单位： | 西南大学农学与生物科技学院;国家马铃薯工程技术研究中心 |
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| 基金项目： | 对发展中国家科技援助项目(KY201904016);西南大学人才引进项目(SWU019008);重庆市技术创新与应用发展专项(cstc2019jscx-msxm X0398)。 |
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| 摘 要： | 利用拟南芥几丁质酶(Chitinase,CHI)基因家族蛋白序列在马铃薯基因组内进行Blast P分析,获得马铃薯CHI家族成员,并对其进行基因结构分析、motif预测、进化树构建、顺式作用元件和组织表达分析。共有26个马铃薯CHI基因成员得到鉴定,其所有成员均含有较少的内含子,其中motif 1~motif 6均含有具有催化功能的GH19结构域。一共检测到166个胁迫或激素响应元件。进化树分析显示,CHI分为6个亚家族。马铃薯几丁质酶基因在不同组织中差异表达并且在水杨酸或茉莉酸处理后诱导表达,暗示水杨酸和茉莉酸对几丁质酶家族有不同的调控作用。
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| 关 键 词： | 马铃薯 几丁质酶 基因 水杨酸 茉莉酸 表达分析 |
Genome-wide Identification and Expression Analysis of Chitinase Gene Family in Potato and Its Response to Exogenous Salicylic Acid and Jasmonic Acid |
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| JIAN Hongju,WAN Mengyuan,FU Yi,DING Yi,JU Xisan,SHANG Lina,LI Hui,WANG Jichun,HU Bogeng,LV Dianqiu.Genome-wide Identification and Expression Analysis of Chitinase Gene Family in Potato and Its Response to Exogenous Salicylic Acid and Jasmonic Acid[J].Acta Horticulturae Sinica,2021(1):83-95. |
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| Authors: | JIAN Hongju WAN Mengyuan FU Yi DING Yi JU Xisan SHANG Lina LI Hui WANG Jichun HU Bogeng LV Dianqiu |
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| Institution: | (College of Agronomy and Biotechnology,State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University,Southwest University,Chongqing 400715,China;National Engineering Research Center for Potato,Dezhou,Shandong 253600,China) |
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| Abstract: | In this study,the chitinase(CHI)gene family protein sequences of Arabidopsis thaliana were used for Blast P analysis in potato genome to obtain the CHI gene members of potato.Gene structure analysis,motif prediction,evolutionary tree construction,cis-acting elements and tissue expression analysis were also carried out.Our results showed that 26 members of potato CHI genes were identified and all Stu Chi gene members contained few introns,and motif 1–motif 6 contained GH19 domain with catalytic function.A total of 166 stress or hormone response elements were detected to participate in the regulation of Stu Chi gene expression.Evolutionary tree analysis showed that CHI were divided into six subfamilies.The quantitative expression of four tissues and the quantitative response to salicylic acid of Stu Chi genes showed that the tissue expression specificity indicated the differentiation of their functions. |
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| Keywords: | potato chitinase gene SA JA expression analysis |
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