不同脂肪源对肉仔鸡肝脏中脂肪代谢相关基因表达的影响

本试验运用牛油、豆油、鱼油三种脂肪酸组成不同的脂肪源设计日粮 ,因而其对肉仔鸡肝脏中FAS、ACC、HMG -COA还原酶基因的mRNA的表达量的影响也存在差异。结果表明富含PUFA的鱼油相对其它脂肪源对此三种基因表达的抑制作用最为明显。运用RT -PCR定量的三种基因的mRNA的量分别比牛油组下降了 5 0 .35 %、4 0 .35 %和81.4 9% ,差异显著 (P <0 .0 5 )。而豆油组中此三种基因的表达相对牛油组下降了 1.4 8%、2 0 .6 9%、2 4 .5 3% ,其抑制效果不如鱼油。

Effect of Differential Fat Sources on Genes Expression of Lipid Metabolism in Liver of Broiler Chickens

The objective of the research was to demonstrate the change in hepatic abundance of FAS,ACC,HMG-CoA reductase mRNAs under beef tallow,soybean oil and fish oil.RT-PCR was applied to evaluate mRNAs abundance.Dietary fish oil reduced by 50.35%、40.35% and 81.49% the hepatic abundance of FAS,ACC,HMG-CoA reductase compared with beef tallow.Fish oil,rich in polyunsaturated fatty acid ,was more effective than soybean oil,which reduced by 1.48%、20.69% and 24.53% respectively compared with beef tallow.