| 冷、热休克法诱导黄颡鱼三倍体的比较研究 |
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| 引用本文： | 宋立民,王卫民,周小云,阎里清,王玉芬. 冷、热休克法诱导黄颡鱼三倍体的比较研究[J]. 水产学报, 2010, 34(5): 768-776. DOI: 10.3724/SP.J.1231.2010.06759 |
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| 作者姓名： | 宋立民 王卫民 周小云 阎里清 王玉芬 |
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| 作者单位： | 华中农业大学水产学院,农业动物遗传育种与繁殖教育部重点实验室,湖北,武汉,430070;中国水产科学研究院北戴河中心实验站,河北,秦皇岛,066100;华中农业大学水产学院,农业动物遗传育种与繁殖教育部重点实验室,湖北,武汉,430070;湖北省漳河水库水产公司,湖北,荆门,448156;中国水产科学研究院北戴河中心实验站,河北,秦皇岛,066100 |
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| 基金项目： | 国家科技支撑计划项目(2006BAD03B07) |
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| 摘 要： | 分别采用冷、热休克抑制第二极体释放的方法诱导黄颡鱼三倍体。结果表明,在卵受精后2min,5℃处理20min,胚胎时期的三倍体率达70%左右,孵化率50%左右,幼鱼时期三倍体(含嵌合体)的检出率为25%,此条件为冷休克处理的优化参数;在卵受精后2min,40℃处理2min,胚胎时期的三倍体诱导率达58%,孵化率为39%,幼鱼时期三倍体(含嵌合体)的检出率为40%,此条件为热休克处理的优化参数。正交分析得出,冷休克条件下起始休克时间是原肠期三倍化率和孵化率的重要影响因子,温度对畸形个体的产生有重要影响;热休克条件下,参考三倍体率、畸形率、孵化期相对存活率三者而言,休克温度均是重要因素。比较观察到冷休克处理组的胚胎受损情况严重,后期的成活率较热休克处理组要低,总体诱导效果逊于热休克处理组。
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| 关 键 词： | 黄颡鱼 冷休克 热休克 人工诱导 三倍体 |
| 收稿时间： | 2009-12-24 |
| 修稿时间： | 2010-03-13 |
Comparative studies in the induction of triploidy for Pelteobagrus fulvidraco by cold and heat shocks |
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| SONG Li-min,WANG Wei-min,ZHOU Xiao-yun,YAN Li-qing,WANG Yu-fen. Comparative studies in the induction of triploidy for Pelteobagrus fulvidraco by cold and heat shocks[J]. Journal of Fisheries of China, 2010, 34(5): 768-776. DOI: 10.3724/SP.J.1231.2010.06759 |
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| Authors: | SONG Li-min WANG Wei-min ZHOU Xiao-yun YAN Li-qing WANG Yu-fen |
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| Affiliation: | SONG Li-min1,2,WANG Wei-min1*,ZHOU Xiao-yun1,YAN Li-qing3,WANG Yu-fen2 (1. Key Laboratory of Agricultural Animal Genetics,Breeding , Reproduction of Ministry of Education,College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China,2. Beidaihe Central Experiment Station,Chinese Academy of Fishery Sciences,Qinhuangdao 066100,3. Fisheries Company of Zhanghe Reservoir,Jingmen 448156,China) |
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| Abstract: | The yellow catfish Pelteobagrus fulvidraco has become a valuable aquaculture species in China and the possible use of sterile triploids is an interesting option for its culture. The optimal conditions for the induction of triploidy for P. fulvidraco by means of cold and heat shocks were investigated and compared in the present study. Three treatment variables were considered: the time after fertilization when the shock was applied,the temperature and the duration of the shock. Ploidy was determined by chrom... |
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| Keywords: | Pelteobagrus fulvidraco cold shock heat shock triploid artificial induction |
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