| 农杆菌介导法将抗草甘膦基因转入玉米自交系 |
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| 引用本文： | 王宏伟,吕颖颖,梁业红,史振声,李博,葛云侠,张世煌. 农杆菌介导法将抗草甘膦基因转入玉米自交系[J]. 玉米科学, 2013, 21(3): 19-23 |
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| 作者姓名： | 王宏伟 吕颖颖 梁业红 史振声 李博 葛云侠 张世煌 |
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| 作者单位： | 沈阳农业大学特种玉米研究所, 沈阳 110161;沈阳农业大学特种玉米研究所, 沈阳 110161;中国农业科学院作物科学研究所, 北京 100081;沈阳农业大学特种玉米研究所, 沈阳 110161;沈阳农业大学特种玉米研究所, 沈阳 110161;沈阳农业大学特种玉米研究所, 沈阳 110161;中国农业科学院作物科学研究所, 北京 100081 |
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| 基金项目： | 辽宁省“十二五”农业攻关计划(2011201018) |
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| 摘 要： | 利用农杆菌介导法将抗草甘膦基因(ssu-G2-aroA)转入优良玉米自交系R18-599和齐319,侵染后的愈伤组织转至潮霉素浓度为10 mg/L的筛选培养基上,继代筛选3轮,每轮3周;得到的抗性愈伤组织在含有潮霉素浓度为3 mg/L的分化培养基上进行分化,R18-599获得30株再生植株,齐319获得18株再生植株。经PCR鉴定后共得到7株转化抗性植株(4株R18-599、3株齐319)。经PCR-Southern及RT-PCR检测结果表明,ssu-G2-aroA基因已稳定整合到了玉米基因组中,并在RNA水平上得到表达。
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| 关 键 词： | 玉米自交系 农杆菌介导 ssu-G2-aroA基因 |
| 收稿时间： | 2012-08-25 |
Agrobacterium Tumefaciens Mediated Transformation of Glyphosate Resistant Gene into Maize Inbred Lines |
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| WANG Hong-wei,L,#; Ying-ying,LIANG Ye-hong,SHI Zhen-sheng,LI Bo,GE Yun-xia and ZHANG Shi-huang. Agrobacterium Tumefaciens Mediated Transformation of Glyphosate Resistant Gene into Maize Inbred Lines[J]. Journal of Maize Sciences, 2013, 21(3): 19-23 |
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| Authors: | WANG Hong-wei,L&# Ying-ying,LIANG Ye-hong,SHI Zhen-sheng,LI Bo,GE Yun-xia ZHANG Shi-huang |
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| Affiliation: | Institute of Special Corn, Shenyang Agricultural University, Shenyang 110161;Institute of Special Corn, Shenyang Agricultural University, Shenyang 110161;Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Institute of Special Corn, Shenyang Agricultural University, Shenyang 110161;Institute of Special Corn, Shenyang Agricultural University, Shenyang 110161;Institute of Special Corn, Shenyang Agricultural University, Shenyang 110161;Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
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| Abstract: | The ssu-G2-aroA gene was transformed into maize inbred lines R18-599 and Qi319 by using Agrobacterium-mediated method. The transformed calli was transferred to the selecting medium with 10 mg/L hygromycin for 9 weeks. Thirty plants of R18-599 and 18 plants of Qi319 were obtained from resistant calli which had been cultured on differentiation medium with 3 mg/L hygromycin. Seven transformants(4 plants R18-599, 3 plants Qi319) were obtained by analyses of PCR, and analyses of PCR-southern and RT-PCR on transformants showed that foreign ssu-G2-aroA gene is integrated into maize genome and is over expressed. The obtained transgenic plants bar boring ssu-G2-aroA. |
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| Keywords: | Maize inbred lines Agrobacterium-mediated ssu-G2-aroA gene |
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