| 盐胁迫下玉米叶片差异蛋白的双向电泳分析 |
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| 引用本文： | 雷震,裴玉贺,赵美爱,郭新梅,宋希云. 盐胁迫下玉米叶片差异蛋白的双向电泳分析[J]. 玉米科学, 2013, 21(3): 82-86 |
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| 作者姓名： | 雷震 裴玉贺 赵美爱 郭新梅 宋希云 |
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| 作者单位： | 青岛农业大学/青岛市主要农作物种质创新与应用重点实验室,山东 青岛,266109 |
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| 基金项目： | 国家科技支撑计划(2011BAD35B01)、青岛市科技支撑计划(12-1-3-15-nsh)、山东省“泰山学者岗位”(作物栽培学与育种学) |
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| 摘 要： | 土壤盐碱化是影响玉米产量的重要非生物因素之一。用200 mmol/L NaCl溶液对耐盐玉米自交系E28进行盐处理，提取叶片可溶性蛋白质进行双向电泳，并用ImageMasterTM 2D 6.00软件分析，探究玉米的耐盐机理。结果表明，盐胁迫处理后共有10个有明显差异的蛋白点，其中4个表达上调，6个表达下调。质谱分析和数据库搜索鉴定出应激反应蛋白和类囊体腔19 KDa蛋白，可能是盐胁迫后植物营养生长及光合作用受到影响的结果。
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| 关 键 词： | 玉米 盐胁迫 双向电泳 质谱分析 |
| 收稿时间： | 2012-11-02 |
Analysis of Soluble Maize Leaf Proteins under Salinity Stress by Two-dimensional Electrophoresis |
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| LEI Zhen,PEI Yu-he,ZHAO Mei-ai,GUO Xin-mei and SONG Xi-yun. Analysis of Soluble Maize Leaf Proteins under Salinity Stress by Two-dimensional Electrophoresis[J]. Journal of Maize Sciences, 2013, 21(3): 82-86 |
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| Authors: | LEI Zhen PEI Yu-he ZHAO Mei-ai GUO Xin-mei SONG Xi-yun |
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| Affiliation: | Qingdao Agricultural University/Qindao Key Lab of Germplasm Innovation and Application of Major Crops, Qingdao 266109, China;Qingdao Agricultural University/Qindao Key Lab of Germplasm Innovation and Application of Major Crops, Qingdao 266109, China;Qingdao Agricultural University/Qindao Key Lab of Germplasm Innovation and Application of Major Crops, Qingdao 266109, China;Qingdao Agricultural University/Qindao Key Lab of Germplasm Innovation and Application of Major Crops, Qingdao 266109, China;Qingdao Agricultural University/Qindao Key Lab of Germplasm Innovation and Application of Major Crops, Qingdao 266109, China |
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| Abstract: | Soil salinization is one of the most important abiotic constraints in maize yield. Seedlings of salinity-tolerant maize inbred line E28 were treated with 200 mmol/L NaCl solution and the soluble proteins of leaves were extracted by TCA/acetone method for two-dimensional gel electrophoresis. Software ImageMasterTM 2D 6.00 was used to analyze the results. There were 4 up-expressed spots and 6 down-expressed spots in total 10 significantly different proteins. MALDI-TOF-MS analysis indicated that there were stress responsive protein and thylakoid lumenal 19 kDa protein, which were considered as the results of changes in maize growth and photosynthesis. |
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| Keywords: | Maize Salinity stress Two-dimensional electrophoresis MALDI-TOF-MS |
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