| 水稻纹枯病生防菌株的筛选及其应用方式的研究 |
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| 引用本文： | 陈立华,沈珊珊,邵孝侯,常婷婷,缪其松,谈俊益. 水稻纹枯病生防菌株的筛选及其应用方式的研究[J]. 中国生态农业学报, 2014, 22(12): 1469-1475 |
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| 作者姓名： | 陈立华 沈珊珊 邵孝侯 常婷婷 缪其松 谈俊益 |
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| 作者单位： | 河海大学水利水电学院/南方地区高效灌排与农业环境教育部重点实验室 南京 210098 |
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| 基金项目： | 江苏省科技支撑项目(BE2011359)、国家自然科学基金项目(2013503811)、中国博士后基金项目(2013M540412)和中央高校基本科研业务费项目(2012B00614)资助 |
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| 摘 要： | 生物防治微生物的应用方式对生防效果具有显著影响。本研究利用分离到的生防菌株T12孢子粉、有机肥吸附菌丝(菌丝吸附)和固体发酵产品(固体发酵)3种水稻纹枯病生防菌的应用方式,研究了3种应用方式对水稻纹枯病发病率、微生物数量以及水稻抗病性酶活性的影响,分析了不同应用方式的生防效果。结果表明,生防菌株T12鉴定为棘孢木霉(Trichoderma asperellum)。孢子粉、菌丝吸附和固体发酵的应用方式都能够防控水稻纹枯病,其中固体发酵应用方式的防效最好,防治率为89.5%。相较于孢子粉处理,应用菌丝吸附和固体发酵的方式能够显著(P0.05)增加水田中立枯丝核菌(Rhizoctonia solani)菌核表面附着的木霉菌数量。水稻移栽20 d测定水稻茎秆上木霉菌数量显示,固体发酵和菌丝吸附应用方式有利于木霉菌在水稻茎秆定殖;水稻移栽60 d、100 d和130 d测定木霉菌数量表明,孢子粉处理木霉菌数量高于固体发酵处理,固体发酵处理高于菌丝吸附处理(P0.05)。水稻生育期内,对照处理立枯丝核菌数量从41 cfu-g-1增加到272 cfu-g-1,孢子粉处理和菌丝吸附处理水稻茎秆上立枯丝核菌数量低于对照处理,但均高于固体发酵处理(P0.05)。测定水稻几丁质酶和β-1,3葡聚糖酶活性的结果显示,3种应用方式都能够诱导水稻产生抗性,相较于孢子粉处理,菌丝吸附和固体发酵处理能够在水稻生育期内持续诱导水稻产生抗性。棘孢木霉T12菌株固体发酵产品的应用方式更有利于对水稻纹枯病的生物防控。
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| 关 键 词： | 棘孢木霉 水稻纹枯病 应用方式 生物防治 固体发酵 |
| 收稿时间： | 2014-07-25 |
| 修稿时间： | 2014-10-21 |
Screening and applying microorganism for biocontrol of rice sheath blight |
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| CHEN Lihu,SHEN Shanshan,SHAO Xiaohou,CHANG Tingting,MIAO Qisong and TAN Junyi. Screening and applying microorganism for biocontrol of rice sheath blight[J]. Chinese Journal of Eco-Agriculture, 2014, 22(12): 1469-1475 |
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| Authors: | CHEN Lihu SHEN Shanshan SHAO Xiaohou CHANG Tingting MIAO Qisong TAN Junyi |
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| Affiliation: | College of Water Conservancy and Hydropower Engineering, Hohai University/Key Laboratory of Efficient Irrigation-Drainage and Agricultural Soil-Water Environment in Southern China, Ministry of Education, Nanjing 210098, China;College of Water Conservancy and Hydropower Engineering, Hohai University/Key Laboratory of Efficient Irrigation-Drainage and Agricultural Soil-Water Environment in Southern China, Ministry of Education, Nanjing 210098, China;College of Water Conservancy and Hydropower Engineering, Hohai University/Key Laboratory of Efficient Irrigation-Drainage and Agricultural Soil-Water Environment in Southern China, Ministry of Education, Nanjing 210098, China;College of Water Conservancy and Hydropower Engineering, Hohai University/Key Laboratory of Efficient Irrigation-Drainage and Agricultural Soil-Water Environment in Southern China, Ministry of Education, Nanjing 210098, China;College of Water Conservancy and Hydropower Engineering, Hohai University/Key Laboratory of Efficient Irrigation-Drainage and Agricultural Soil-Water Environment in Southern China, Ministry of Education, Nanjing 210098, China;College of Water Conservancy and Hydropower Engineering, Hohai University/Key Laboratory of Efficient Irrigation-Drainage and Agricultural Soil-Water Environment in Southern China, Ministry of Education, Nanjing 210098, China |
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| Abstract: | |
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| Keywords: | Trichoderma asperellum Sheath blight Application method Biocontrol Solid-state fermentation |
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