RAPD技术在石榴品种分类上的应用

以55个石榴栽培品种为试验材料,利用15条多态性好的随机引物进行RAPD分析,共扩增出125条带,其中多态性条带92条,多态性百分率73.6%,说明品种间有变异。应用TFPGA软件计算55个石榴品种间的Nei’s遗传距离,并用UPGMA法构建聚类图,将55个石榴品种分为4个类群,从DNA水平上揭示了石榴品种之间的亲缘关系。结果表明,采用UPGMA法聚类的结果与形态上的分类存在着一定的差异,即与根据花色和果味进行的分类没有相关性而与瓣型进行的分类有一定的相关性。

Studies on classification of pomegranate cultivars by RAPD analysis

RAPD (random amplified polymorphic DNA) analysis was applied to 55 pomagranate cultivars by using 15 random primers and a total of 125 bands were generated, of which 92 were polymorphic bands (the percentage of polymophic loci,PPB=73.6%).The results revealed that there was variance in the 55 cultivars. According to the results of RAPD amplification, which were analyzed to Nei’s genetic distance by TFPGA software, the clustering dendrogram was constructed by UPGMA method. These 55 cultivars were classified into 4 major groups. At the same time, the genetic relationships between the 55 cultivars were also revealed. The results of RAPD had some differences with morphological classification. The results do not support the morphological classification based on the flower color and fruit taste but have relevance to that of the flower petal.