Methane-oxidizing activities and methanotrophic populations associated with wetland rice plants |
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Authors: | I Watanabe T Hashimoto A Shimoyama |
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Institution: | (1) 6-58-18, Jindaiji-Kita, Chofu, Tokyo, 182, Japan e-mail: it6i-wtnb@asahi-net.or.jp, JP |
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Abstract: | Acetylene up to 500 μl l–1 did not affect methane formation in anoxic soil up to 12 h, but further incubation for 1 week showed strong inhibition of
methanogenesis. To ascertain the extent of the oxidation of methane produced from rice-planted pots, the effect of acetylene
on methane emission was studied. Two rice varieties (Toyohatamochi and Yamahikari) were grown in a greenhouse in submerged
soil in pots. At about maximum tillering, heading, and grain-forming stages, methane fluxes were measured. Flux measurement
was performed for 3 h from 6 pm, then acetylene at 100 μl l–1 was added to some of the pots. At 6 a.m. the following day, methane fluxes were again measured for 3 h. Only at maximum tillering
stage of the variety Toyohatamochi was a significant increase (1.4 times) in methane flux caused by acetylene observed, whereas
in the other treatments no significant increase in methane fluxes by acetylene could be defected. To ascertain the activity
of methane monooxygenase (MMO), propylene oxide (PPO) formation from propylene was measured with excised roots and a basal
portion of stems of the rice plants grown on the submerged soil. A level of 0.1–0.2 μmol PPO h–1 plant–1 was recorded. The roots showed the highest PPO formation per gram dry matter, followed by basal stems. Methane oxidation
was roughly proportional to PPO formation. Soluble MMO-positive methanotroph populations were measured by plate counts. The
number of colony-forming units per gram dry matter was 106–105 in roots, and 104–103 in basal stems. These results indicate the possibility of methane oxidation in association with wetland rice plants.
Received: 26 October 1995 |
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Keywords: | Methane Wetland rice soils Oryza sativa Methane oxidation Acetylene Propylene oxide Methanotrophs Rhizosphere |
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