免疫微球凝集试验在鸡传染性法氏囊病免疫检测中的应用

The Application of Immunomicrosphere Agglutination Test to Immunodetection of Infectious Bursal Disease of Chicken

Using carboxyl polystrene microspheres(CPM)as carriers andthrough covalently connecting the cell cultures(sensitizing antigen)of infect-ious bursal disease virus(IBDV)of chicken to CPMs via the reaction oflethy-1-3-(3-diimethylaminopropyl)-carbodimide(EDC),the test has successfullyprepared the immunomicrospheres for the detection of antibodies against IBDVof chicken.The comparative detection of immunomicrosphere agglutina-tiontest(IMSAT)and agar-gel precipitin test(AGP)for serum antibodies againstIBDV of chicken has unclosed that IMSAT is of strong specificity,and it issimple in operation and fast in determination.The examination of serum anti-bodis of immunized chicken by two methods described above shows that the combining Immunization of Newcastle disease(ND)vaccine and IBD modifiedvirus vaccine of chicken is feasible,and can obtain the same effect as theindividual inoculation with modified IBDV vaccine.The authors also point outthe ideal immunoprocedure of IBD of chicken and detecting methods throughstudying the growth and decline of IBD maternal antibodies of young chicken.