(1) CPI Division, Rothamsted Research, Harpenden, AL5 2JQ, UK
Abstract:
Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration in tissue culture have been further investigated
and modified. This paper reviews the current methodology literature describing Agrobacterium transformation of wheat and provides a complete protocol that we have developed and used to produce over one hundred transgenic
lines in both spring and winter wheat varieties.