| 柑橘种间六倍体体细胞杂种开花及其花粉育性 |
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| 引用本文： | 郭文武,陈春丽,邓于洋,邓秀新.柑橘种间六倍体体细胞杂种开花及其花粉育性[J].园艺学报,2007,34(1):71-75. |
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| 作者姓名： | 郭文武 陈春丽 邓于洋 邓秀新 |
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| 作者单位： | (华中农业大学作物遗传改良国家重点实验室, 武汉430070) |
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| 基金项目： | 国家自然科学基金;教育部资助项目 |
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| 摘 要： | 二倍体锦橙+种间四倍体体细胞杂种HR (哈姆林甜橙+ 粗柠檬) 的种间六倍体体细胞杂种植株移入温室7年后(即2004年) , 有1株六倍体首次开花, 花粉染色活力40.8% , 花粉萌发率3.7%;2006年该植株再次开花, 花粉染色活力为20.3%。由于自然界中没有柑橘六倍体, 本研究是人工的方法创造出可育的柑橘六倍体体细胞杂种植株的首例报道。
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| 关 键 词： | 柑橘 六倍体 体细胞杂种 开花 育性评价 |
| 文章编号： | 0513-353X（2007）01-0071-04 |
| 收稿时间： | 2006-06-11 |
| 修稿时间： | 2006-06-112006-11-28 |
Flowering and Pollen Fertility of an Interspecific Citrus Hexaploid Somatic Hybrid |
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| GUO Wen-wu,CHEN Chun-li,DENG Yu-yang,DENG Xiu-xin.Flowering and Pollen Fertility of an Interspecific Citrus Hexaploid Somatic Hybrid[J].Acta Horticulturae Sinica,2007,34(1):71-75. |
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| Authors: | GUO Wen-wu CHEN Chun-li DENG Yu-yang DENG Xiu-xin |
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| Institution: | 1.Institute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China;2.Department of Respiratory Disease, Shanghai East Hospital, Shanghai 200120, China. E-mail: leihan@sina.com |
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| Abstract: | Interspecific hexaploid somatic hybrid between Jincheng orange and HR (Hamlin orange Rough lemon) regenerated via electrofusion flowered seven years after transplantation in greenhouse. Flow cytometry analysis further confirmed its true hexaploid nature. Its pollen stainability and germinability was 40.8% and 3.7%, respectively. In 2006, the hexalpoid plant flowered again and its pollen stainability was 20.3%. Since there is no natural citrus hexaploid plant, this is the first report of fertile hexaploid somatic hybrid plant in Citrus. |
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| Keywords: | Citrus Hexaploid Somatic hybrid Flowering Fertility evaluation |
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