Vaccination of bovines with recombinant Boophilus Yolk pro-Cathepsin |
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| Authors: | Leal Alexandre T Seixas Adriana Pohl Paula C Ferreira Carlos A S Logullo Carlos Oliveira Pedro L Farias Sandra E Termignoni Carlos da Silva Vaz Itabajara Masuda Aoi |
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| Institution: | Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gon?alves, 9500, Prédio 43421, Porto Alegre RS 91501-970, Brazil. |
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| Abstract: | Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization. |
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