| 应用TaqMan MGB探针检测美澳型核果褐腐病菌 |
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| 引用本文： | 程颖慧,王颖,章桂明,陈枝楠,李小焦.应用TaqMan MGB探针检测美澳型核果褐腐病菌[J].广东农业科学,2009(7):196-198. |
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| 作者姓名： | 程颖慧 王颖 章桂明 陈枝楠 李小焦 |
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| 作者单位： | 1. 深圳出入境检验检疫局,广东,深圳,518010
2. 重庆大学生物工程学院,重庆,400044 |
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| 基金项目： | 国家质检总局科研项目 |
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| 摘 要： | 以美澳型核果褐腐病菌的6个菌株以及核果褐腐病菌一个和欧洲种仁果褐腐病菌这两种近似种为研究对象,通过对ITS区序列的比对分析,设计了美澳型核果褐腐病菌的TaqMan MGB实时荧光PCR引物和探针,建立了美澳型核果褐腐病菌的TaqMan MGB实时荧光PCR检测方法.设计了这3种近似种的通用引物和探针,用于检测过程中进行质量控制.
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| 关 键 词： | 美澳型核果褐腐病菌 TaqMan.MGB探针 实时荧光PCR |
Detection of Monilinia fructicola (winter) honey by 5'-nucleaseassays using TaqMan MGB probe |
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| CHENG Ying-hui,WANG Ying,ZHANG Gui-ming,CHEN Zhi-nan,LI Xiao-jiao.Detection of Monilinia fructicola (winter) honey by 5'-nucleaseassays using TaqMan MGB probe[J].Guangdong Agricultural Sciences,2009(7):196-198. |
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| Authors: | CHENG Ying-hui WANG Ying ZHANG Gui-ming CHEN Zhi-nan LI Xiao-jiao |
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| Abstract: | The 3 species were studied by real-time PCR approach, including 6 strains of M. fructicola and their similar species, M. laxa, M. fructigena. The primers and TaqMan MGB probes were designed for detection of M. fructicola by analysis of ITS sequences differences. The common primers and probe was also used to control the quality of the reaction. |
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| Keywords: | Monilinia fructieola (Winter) Honey TaqMan MGB real time fluorescent PCR |
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