| 枣组织培养中抗生素对污染的抑制作用 |
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| 引用本文： | 邢东方,尚霄丽,冯建灿,李继东,喻晓敏. 枣组织培养中抗生素对污染的抑制作用[J]. 经济林研究, 2010, 28(1): 26-29,68 |
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| 作者姓名： | 邢东方 尚霄丽 冯建灿 李继东 喻晓敏 |
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| 作者单位： | 河南农业大学,河南郑州,450002 |
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| 基金项目： | 河南省杰出青年计划项目“河南枣种质资源分子评价与抗虫转基因新品种培育研究”(084100410021) |
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| 摘 要： | 为解决枣组织培养中初代培养阶段外植体污染问题,以灰枣1年生嫁接苗枣头茎段为外植体,研究了4种抗生素及其不同质量浓度对灰枣组织培养中污染的抑制作用。结果表明:头孢霉素抑制效果最好,150mg/L、200mg/L质量浓度处理污染率均为0,链霉素次之,卡那霉素和青霉素G钠盐对污染的抑制效果均较差;链霉素对枣腋芽的萌发有抑制作用,培养基添加200mg/L头孢霉素时腋芽细弱,叶片黄化。因此在枣组织培养中可使用150mg/L头孢霉素来抑制污染。
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| 关 键 词： | 灰枣 组织培养 抗生素 污染 |
Effects of antibiotics on controlling contamination during tissue culture in Ziziphus jujuba.Mill |
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| XING Dong-fang,SHANG Xiao-li,FENG Jian-can,LI Ji-dong,YU Xiao-min. Effects of antibiotics on controlling contamination during tissue culture in Ziziphus jujuba.Mill[J]. Economic Forest Researches, 2010, 28(1): 26-29,68 |
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| Authors: | XING Dong-fang SHANG Xiao-li FENG Jian-can LI Ji-dong YU Xiao-min |
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| Affiliation: | Henan Agricultural University;Zhengzhou 450002;Henan;China |
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| Abstract: | In order to solve the problem of contamination during tissue culture in Ziziphus jujuba., using one-year-old grafted seedings stems in Z. jujuba. Huizao as explants, effects of four kinds of antibiotics on controlling contamination were studied. The results showed that cephalosporin had the best effect on controlling contamination, and the contamination rates were 0 when the concentrations were 150 mg/L and 200 mg/L. Effect of streptomycin took the second place, and both kanamycin and penicillin G sodium ha... |
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| Keywords: | Ziziphus jujuba. Huizao tissue culture antibiotic contamination |
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