基于玉米87-1综3单片段代换系的穗长QTL分析

以优良玉米自交系综3为供体、87-1为受体,利用回交和SSR标记辅助选择相结合的方法,获得了51份纯合的染色体单片段代换系(Single Segment Substitution Line,SSSL),每个SSSL内只含有一个来源于供体的染色体片段。51个代换片段不均匀分布在玉米10条染色体上,代换片段长度在1.75～172.45 cM之间,平均长度为24.44cM。覆盖玉米基因组的总长度为933.90 cM,覆盖率为40.20%。2008年在郑州利用17份SSSL材料及在三亚利用39份SSSL材料对玉米穗长进行了1年2点的表型鉴定,共检测到20个穗长QTL,加性效率百分率在-12.10%～19.18%之间。结果表明,这些单片段代换系存在较大的遗传变异,是用于玉米复杂性状的QTL鉴定、基因克隆及功能分析的理想材料。

QTL Mapping for Ear Length Based on Chromosome Single Segment Substitution Lines of 87-1 Zong3 in Maize

A wide population consisting of 51 chromosome single-segment substitution lines(SSSLs) were developed from an advanced backcross procedure with the marker-assisted selection of simple sequence repeat(SSR) markers using the elite maize inbred line 87-1 as the recipient and Zong3 as the donor. Each SSSL contains a single substituted chromosome segment derived from donor Zong3 in the genetic background of 87-1. The total size of the substituted segments in the SSSL population was 1 246.55 cM, and the genome covered length was 933.90 cM, with a covered ratio of 40.2%. The length of these substituted segments ranged from 1.75 - 172.45 cM, with an average of 24.44 cM. To evaluate the potential application of these SSSLs for QTL detection, phenotypic variations of ear length of 39 SSSLs were observed in two different places. Twenty-three QTLs for ear length were identified and mapped on substituted segment, which the percentages of additive effects varied from -12.10% to 19.18%. The results demonstrated that SSSLs are excellent genetic materials for QTL identification of dissect complex traits, gene cloning and functional genomics.