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十氯酮污染土壤上根茎作物收割部分的污染状况
作者姓名:Y. M. CABIDOCHE  M. LESUEUR-JANNOYER
作者单位:Research Unit 1321 Agrosyst¨¨mes Tropicaux, INRA, Duclos, 97170 Petit-Bourg (France);Pôle de Recherche Agro-Environnementale de Martinique, CIRAD, Petit-Morne, BP 214, 97285 Lamentin (France);UR103 Fonctionnement Agroécologique et Performances des Systèmes de Culture Horticoles, CIRAD, TA B-103/PS4, Bd de la Lironde, 34389 Montpellier Cedex 5 (France)
基金项目:Supported by the National Natural Science Foundation of China(Nos.50978122 and U0933002)
摘    要:A bacterial strain, Arthrobacter oxydans (B4), capable of degrading benzoa]pyrene (BaP) in water body, was isolated from a polycyclic aromatic hydrocarbons-contaminated site. Effects of different factors, such as reaction time, pH value, temperature and organic nutrients, on BaP biodegradation by the strain B4 were studied. After 5 d treatment, the concentration of BaP in mineral salts medium was reduced to 0.318 mg L-1 , compared to the initial concentration of 1.000 mg L-1 . There was a process of acid formation during the degradation with pH falling from initial 7.01 to 4.61 at 5 d, so keeping the water body under slightly alkaline condition was propitious to BaP degradation. Strain B4 efficiently degraded BaP at 20 to 37 ℃ with addition of organic nutrients. The biodegradation and transformation of BaP mainly occurred on cell surfaces, and extracellular secretions played an important role in these processes. Fourier transform infrared spectroscopy and gas chromatograph-mass spectrometer analyses of metabolites showed that ring cleavage occurred in the BaP degradation process and the resulting metabolically utilizable substrates were generated as sole carbon sources for B4 growth. Furthermore, mineralization extent of metabolites was verified by determining the total organic carbon and inorganic carbon in the degradation system.

关 键 词:degradation  mechanism  metabolites  microbial  transformation  polycyclic  aromatic  hydrocarbon
收稿时间:24 February 2012

Contamination of harvested organs in root crops grown on chlordecone-polluted soils
Y. M. CABIDOCHE,M. LESUEUR-JANNOYER.Contamination of harvested organs in root crops grown on chlordecone-polluted soils[J].Pedosphere,2012,22(4):562-571.
Authors:Y M CABIDOCHE and M LESUEUR-JANNOYER
Institution:Department of Environmental Engineering, Jinan University, Guangzhou 510632 (China) ; Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632 (China);Department of Environmental Engineering, Jinan University, Guangzhou 510632 (China) ; Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632 (China);Department of Environmental Engineering, Jinan University, Guangzhou 510632 (China) ; Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632 (China);Department of Environmental Engineering, Jinan University, Guangzhou 510632 (China) ; Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632 (China);Department of Environmental Engineering, Jinan University, Guangzhou 510632 (China) ; Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632 (China);Department of Environmental Engineering, Jinan University, Guangzhou 510632 (China) ; Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632 (China);Department of Environmental Engineering, Jinan University, Guangzhou 510632 (China) ; Key Laboratory of Water/Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632 (China)
Abstract:A bacterial strain, Arthrobacter oxydans (B4), capable of degrading benzoa]pyrene (BaP) in water body, was isolated from polycyclic aromatic hydrocarbons-contaminated site. Effects of different factors, such as reaction time, pH value, temperature and organic nutrients, on BaP biodegradation by the strain B4 were studied. After 5 d treatment, the concentration of BaP in mineral salts medium was reduced to 0.318 mg L-1, compared to the initial concentration of 1.000 mg L-1. There was a process of acid formation during the degradation with pH falling from initial 7.01 to 4.61 at 5 d, so keeping the water body under slightly alkaline condition was propitious to BaP degradation. Strain B4 efficiently degraded BaP at 20 to 37 oC with addition of organic nutrients. The biodegradation and transformation of BaP mainly occurred on cell surfaces, and extracellular secretions played an important role in these processes. Fourier transform infrared spectroscopy and gas chromatograph-mass spectrometer analyses of metabolites showed that ring cleavage occurred in the BaP degradation process and the resulting metabolically utilizable substrates were generated as sole carbon sources for B4 growth. Furthermore, mineralization extent of metabolites was verified by determining the total organic carbon and inorganic carbon in the degradation system.
Keywords:degradation  mechanism  metabolites  microbial transformation  polycyclic aromatic hydrocarbon
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