番茄抗黄化曲叶病毒病基因的AFLP分子标记

用番茄抗黄化曲叶病毒病品系‘T0727’与高感黄化曲叶病毒病品系‘T9179’配制杂交组合,接种鉴定其F₁代及F₂代分离群体的黄化曲叶病毒病发生情况。用64对EcoRⅠ/MseⅠ引物组合对‘T0727’、‘T9179’两个亲本及其F₁代和F₂代抗病和感病基因池进行AFLP分析,共扩增出4 023条可分辨的条带,其中3条为稳定的差异。用‘T0727’和 ‘T9179’杂交产生的F2代分离群体对3个特异条带与目的基因的遗传连锁性进行分析,发现特异条带E-ACC/M-CAG与抗黄化曲叶病毒病基因紧密连锁,二者之间的遗传距离为9.5 cM。将E-ACC/M-CAG片段回收、克隆和测序,成功地将其转化为SCAR标记,暂定名为Afty-196,可以用于对番茄黄化曲叶病毒病基因的标记辅助选择。

Identification of AFLP markers linked to TYLCVD-resistance gene in tomato

F₁ and F₂ progeny of cross combination with TYLCVD-resistant tomato material T0727 and susceptible material T9179 were inoculated. With 64 EcoRⅠ/MseⅠ primer combinations, AFLP analysis was performed on two parents, their F₁ and F₂ resistant and susceptible bulks. A total of about 4 023 distinguishable bands were amplified, three of which were stable. Genetic linkage analysis of the three polymorphic DNA fragments with the resistance genes was tested in the F₂ segregating population derived from the cross with T0727 and T9179. The DNA fragment E-ACC/M-CAG was found to be closely linked to one of the TYLCVD-resistant genes, with a genetic distance of 9.5 cM, which was tentatively named Afty-196. The cloned fragment E-ACC/M-CAG was sequenced and then successfully converted to a SCAR marker, which can be used in marker-assisted selection for tomato resistant to tomato yellow leaf curl virus disease．