| 重离子束注入小麦胚乳诱发的突变效应 |
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| 引用本文： | 颉红梅,卫增泉,李兴林,王浩瀚,赵莲芝,谢忠奎. 重离子束注入小麦胚乳诱发的突变效应[J]. 核农学报, 2004, 18(2): 93-96 |
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| 作者姓名： | 颉红梅 卫增泉 李兴林 王浩瀚 赵莲芝 谢忠奎 |
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| 作者单位： | 中国科学院近代物理研究所,甘肃,兰州,730000;甘肃省张掖地区农科所,甘肃,张掖,734000;中国科学院寒区旱区环境与工程研究所,甘肃,兰州,730000 |
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| 基金项目： | 甘肃省自然科学基金(SJ970607)和中国科学院寒区旱区环境与工程研究所创新项目(210069) |
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| 摘 要： | 采用 75MeV u16O8+ 离子 (降能至 36MeV u ,使其在胚乳中的射程约为 2 4mm ) ,从种子顶部垂直注入胚乳 ,通过“嫁接”技术后进行萌发。处理后观测结果表明 ,当代根尖细胞染色体的微核率和畸变率与注入剂量呈正相关 ;测定了当代抗氧化酶活性、丙二醛 (MDA)含量和蛋白质含量的变化 ;经多代大田培育获得了矮杆与各种穗型的变异 ,且具有遗传性。结果显示离子注入胚乳不仅对生物的修复系统有较大影响 ,而且能够诱发后代突变。
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| 关 键 词： | 离子注入 胚乳 突变 |
| 文章编号： | 1000-8551(2004)02-093-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2002-10-18 |
MUTAGENIC EFFECTS OF ENDOSPERM OF Triticum aestivum IMPLANTED BY HEAVY ION BEAMS |
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| XIE Hong-mei LI Xing-lin WEI Zeng-quan WANG Hao-han ZHAO Lian-zhi XIE Zhong-kui. MUTAGENIC EFFECTS OF ENDOSPERM OF Triticum aestivum IMPLANTED BY HEAVY ION BEAMS[J]. Acta Agriculturae Nucleatae Sinica, 2004, 18(2): 93-96 |
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| Authors: | XIE Hong-mei LI Xing-lin WEI Zeng-quan WANG Hao-han ZHAO Lian-zhi XIE Zhong-kui |
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| Affiliation: | XIE Hong-mei 1 LI Xing-lin 1 WEI Zeng-quan 1 WANG Hao-han 2 ZHAO Lian-zhi 2 XIE Zhong-kui 3 |
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| Abstract: | MeV/u 16 O 8 ions (degraded to 36MeV/u) were used to implant into endosperm about 2.4mm on top of the seeds. Germination started after a `grafting' technique was employed. Chromosomal aberration frequency and micronucleus frequency of the root-tip cells in M 0 were measured. The results indicate that the frequencies were proportional to implanted dose. Antioxidant enzyme activity, MDA content and protein content of present generation M 0 were assayed. Farm culture was carried out in many generations. Short-stem and various variation of ear-type were obtained and the variation possess heredity. It showed that the endosperm implanted by the ions not only affected biological repair system, but also induced the mutation of offspring. |
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| Keywords: | ion implantation endosperm mutation |
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