| 胰岛素、胰高血糖素对体外培养脂肪细胞FAS mRNA表达的影响 |
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| 引用本文： | 王娅,郑健宇,邓俊良,左之才,彭西,任志华,胡延春. 胰岛素、胰高血糖素对体外培养脂肪细胞FAS mRNA表达的影响[J]. 兽医大学学报, 2013, 0(9): 1454-1457 |
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| 作者姓名： | 王娅 郑健宇 邓俊良 左之才 彭西 任志华 胡延春 |
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| 作者单位： | [1]四川农业大学动物医学院,四川雅安625014 [2]宜宾市翠屏区动物防疫检疫中心,四川宜宾644000 |
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| 基金项目： | 教育部《长江学者和创新团队发展计划》创新团队资助项目（IRTO848）; 四川省教育厅重点资助项目（10ZA048）; 四川农业大学优秀学士毕业论文培育计划项目 |
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| 摘 要： | 为阐明胰岛素、胰高血糖素与脂肪酸合成酶（FAS）的关系,在体外培养良好的脂肪细胞中添加不同浓度的胰岛素（INS）、胰高血糖素（GLU）,每个激素设置6个浓度梯度,培养12h后,通过荧光定量PCR技术,观测胰岛素、胰高血糖素对FAS mRNA丰度的影响。结果显示,胰岛素促进了FAS mRNA的表达,而胰高血糖素抑制了FAS mRNA的表达。结果表明,胰岛素和胰高血糖素能直接调控肝细胞中FAS mRNA的表达。
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| 关 键 词： | 胰岛素 胰高血糖素 脂肪细胞 FAS mRNA |
Effects of INS and GLU on abundance of FAS mRNAin vitro culture bovine adipocyte |
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| WANG Ya;ZHENG Jian-yu;DENG Jun-liang;ZUO Zhi-cai;PENG Xi;REN Zhi-hua;HU Yan-chun. Effects of INS and GLU on abundance of FAS mRNAin vitro culture bovine adipocyte[J]. , 2013, 0(9): 1454-1457 |
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| Authors: | WANG Ya ZHENG Jian-yu DENG Jun-liang ZUO Zhi-cai PENG Xi REN Zhi-hua HU Yan-chun |
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| Affiliation: | WANG Ya;ZHENG Jian-yu;DENG Jun-liang;ZUO Zhi-cai;PENG Xi;REN Zhi-hua;HU Yan-chun;College of Veterinary Medicine,Sichuan Agricultural University;Cuiping Animal Epidemic Prevention and Quarantine Center of Yibin; |
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| Abstract: | To clarify regulation of INS and GLN on the mRNA expression of FASin vitro neonatal calf adipocytes,different concentrations of INS and GLN were added respectively into media of primary monolayer culture calf adipocytes.After 12hcultured,mRNA abundance of FAS in the adipocytes was detected by FQ-PCR respectively.The results showed that INS plays a stimulating a role in abundance of FAS mRNA,GLU plays a inhibitory role. |
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| Keywords: | INS GLU adipocytes FAS mRNA |
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