边鸡MSTN基因C.234位点对生长性状的遗传效应分析 |
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引用本文: | 张跟喜,曹宁贤,丁馥香,王金玉,戴国俊,谢恺舟,张丽.边鸡MSTN基因C.234位点对生长性状的遗传效应分析[J].兽医大学学报,2013(9):1470-1474. |
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作者姓名: | 张跟喜 曹宁贤 丁馥香 王金玉 戴国俊 谢恺舟 张丽 |
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作者单位: | [1]扬州大学动物科学与技术学院,江苏扬州225009 [2]山西省畜禽繁育工作站,山西太原030032 [3]山西省农业科学院畜牧兽医研究所,山西太原030032 |
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基金项目: | 国家自然科学基金资助项目(31201793); 江苏高校优势学科建设工程资助项目; 扬州大学高层次人才科研启动基金; 国家肉鸡产业技术体系资助项目(nycytx-42-G1-05); 山西省科技攻关资助项目(20100311041) |
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摘 要: | 采用PCR-RFLP技术检测连续2个世代边鸡群体肌肉生长抑制素基因外显子1的多态性,分析了多态位点对边鸡生长性状的遗传效应。结果表明,边鸡MSTN基因的C.234位点存在多态性,在外显子1编码区的234bp处发生G→A的碱基突变,产生了GG、GA和AA 3种基因型。关联分析表明,无论是一世代还是二世代的边鸡群体,在6~16周龄时,AA和GA基因型边鸡的体质量均显著或极显著的高于GG基因型(P〈0.05或P〈0.01)。结果提示,MSTN基因外显子1的C.234位点突变对边鸡的生长性状具有显著的遗传效应,初步推断该位点可用于边鸡生长性状的标记辅助选择。
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关 键 词: | 肌肉生长抑制素基因 边鸡 PCR-RFLP 生长性状 |
Genetic effect of the C.234 locus of the MSTN gene on growth traits in Bian chicken |
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Authors: | ZHANG Gen-xi;CAO Ning-xian;DING Fu-xiang;WANG Jin-yu;DAI Guo-jun;XIE Kai-zhou;ZHANG Li |
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Institution: | ZHANG Gen-xi;CAO Ning-xian;DING Fu-xiang;WANG Jin-yu;DAI Guo-jun;XIE Kai-zhou;ZHANG Li;College of Animal Science and Technology,Yangzhou University;Shanxi Livestock and Poultry Breeding Station;Institute of Animal Husbandry and Veterinary,Shanxi Academy of Agricultural Sciences; |
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Abstract: | This study aimed to investigate the relationship between the MSTN gene and chicken growth traits,and search the marker correlated with chicken growth traits.Single nucleotide polymorphism(SNP)in exon 1 of the myostatin gene was detected by PCR-RFLP in Bian chicken of two consecutive generations.The results showed that C.234 locus displayed polymorphic.A G→ A mutation at the position of the 234 bp of coding region in exon 1 was found,which resulted in 3 genotypes(GG,GA,and AA).Correlation analysis showed that,no matter for generation one or generation two,female Bian chickens of genotypes AA and GA had significantly higher body weight than those of genotype GG(P0.05or P0.01)from 6 to 16 weeks of age.These results suggest that the C.234 locus in exon 1 of the myostatin gene has significantly genetic effect on growth traits,and could be used as a genetic marker for growth traits in Bian chicken. |
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Keywords: | myostatin(MSTN) Bian chicken PCR-RFLP growth traits |
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