Expression of the chicken lysozyme gene in potato enhances resistance to infection byErwinia carotovora subsp.atroseptica |
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Authors: | Carolina Serrano Patricio Arce-Johnson Hebert Torres Marlene Gebauer Mónica Gutierrez Mauricio Moreno Xavier Jordana Alejandro Venegas Julio Kalazich Loreto Holuigue |
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Affiliation: | 1. Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, P.O. Box 114-D, Santiago, Chile 2. Centro Regional de Investigation Remehue, Instituto de Investigaciones Agropecuarias (INIA), P.O. Box 24-O, Osorno, Chile 3. Facultad de Agronomía, Pontificia Universidad Católica de Chile, P.O. Box 306, Santiago, Chile
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Abstract: | Infection of potato plants and tubers with the bacteriumErwinia carotovora subsp.atroseptica produces blackleg and soft rot diseases, which cause significant losses to crops and stored potatoes. In order to obtain resistance against this bacterium, the genechly encoding the enzyme lysozyme from chicken was introduced into potato plants (cv. Desirée) viaAgrobacterium- mediated transformation. Sixty-three and 69 transgenic potato clones were evaluated in the greenhouse for resistance to blackleg and soft rot diseases, respectively. Results reported in this paper indicate that 21%-29% of the potato clones showed increased resistance to infection by the bacteriumE. c. subsp.atroseptica T7, as revealed by a reduced severity of blackleg or soft rot symptoms. Nine clones showing different levels of resistance were selected for further molecular analysis. The number of copies of the transgene integrated in the plant genome of these clones was estimated by Southern blot analysis. The level of transgene expression, detected by Northern blot analysis, correlated with the level of resistance detected in these clones. |
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