玉米地方品种Glb1基因克隆及其遗传多样性分析

【目的】了解我国西南地区不同玉米地方品种的遗传多样性,为合理利用这些玉米种质资源提供参考。【方法】以西南地区12份玉米地方品种为材料,对Glb1基因片段进行克隆、测序及序列比对,分析其遗传多样性。【结果】12份玉米材料Glb1基因序列长度为1138-1149 bp,其序列一致性为96.21%。12份材料Glb1基因序列间存在较多的变异位点,大于等于3 bp的插入/缺失有8个,主要位于第81-84、122-128、161-164、368-371、428-442、668-680、966-976和1003-1011 bp。不同玉米地方品种材料间的遗传相似系数为96.50%-100.00%,平均为98.58%。根据聚类分析结果可将12份材料分为五大类,且来源于同一地区的玉米材料趋向于聚为一类。【结论】Glb1基因具有较高的保守性,所选材料间Glb1基因序列存在一定的遗传多样性,利用Glb1基因序列可作为鉴定不同玉米地方品种的一种理想分子标记。

Cloning of Glb1 gene and its genetic diversity in different maize landraces

【Objective】The present experiment was conducted to study genetic diversity and relationship among different maize landraces collected from Southwest China in order to provide references for reasonable application of these germplasm resources. 【Method】The 12 accessions of maize landrace collected from Southwest China were used as materials to analyze their genetic diversity by clone and sequence alignment of Glb1 gene. 【Result】The length of Glb1 gene sequence in 12 maize landraces changed from 1138 to 1149 bp with 96.21% of consistency. There existed rich variable sites among Glb1 gene sequences of 12 landraces. Out of which, eight insertions/deletions ≧ 3 bp were located at the loci of 81-84, 122-128, 161-164, 368-371, 428-442, 668-680, 966-976 and 1003-1011 bp,respectively. The coefficient of genetic similarity among 12 landraces ranged from 96.50% to 100.00%, with an average of 98.58%. The cluster analysis results showed that 12 maize landraces could be classified into five groups, and those landraces came from the same geographical distribution were tended to cluster into one group. 【Conclusion】Glb1gene is highly conservative,and its sequence showed certain genetic diversity among different maize landraces. Therefore, Glb1 gene sequence could be used as an ideal molecular marker for identifying different maize landraces.