| 牛结核分枝杆菌PCR检测方法的建立 |
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| 引用本文： | 刘忠发,张华,李涛,沙祖敏,杜春林,姜德荣,周碧君,程振涛.牛结核分枝杆菌PCR检测方法的建立[J].贵州畜牧兽医,2014(2):1-4. |
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| 作者姓名： | 刘忠发 张华 李涛 沙祖敏 杜春林 姜德荣 周碧君 程振涛 |
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| 作者单位： | [1]贵州大学动物科学学院,贵州贵阳550025; [2]贵州省动物疫病预防控制中心,贵州贵阳550008 |
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| 基金项目： | 贵州大学大学生“SRT计划”项目“奶牛结核病诊断方法比较研究”贵大SRT字（2012）072号;贵阳市科技计划项目“奶牛结核病综合防控技术创新集成与推广”筑科合同[2012207]号;贵州省农业攻关项目“贵州省主要人畜共患病综合防控技术研究”黔科合NY字[2011]3061号 |
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| 摘 要： | 随着牛结核病疫情日趋严重,快速、高效的分子生物学检测方法建立显得十分必要.试验设计合成了牛结核分枝杆菌特异性引物,优化反应条件,建立了牛分枝杆菌PCR检测方法.结果显示,所建立的牛结核分枝杆菌PCR检测方法可有效检测牛结核分枝杆菌,在模板浓度、引物浓度和退火温度分别为1.32 μg/mL、0.5 μm/L、54℃时最佳;性能评估显示,该方法具有较好的特异性、敏感性和临床应用性,为我省牛结核病的综合防控提供关键技术.
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| 关 键 词： | 牛结核分枝杆菌 PCR检测 方法建立 |
Development of PCR Assay for Detection of Bacillus Tuberculosis Bovis |
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| Institution: | Liu Zhongfa, Zhang Hua , Li Tao , Sha Zumin , Du Chunlin , Jiang Derong , Zhou Bijun , Cheng Zhentao ( 1. College of Animal Science, Guizhou university, Guiyang Guizhou 550025, China; 2. Animal Disease Prevention and Control Center in Guizhou Province, Guiyang Guizhou 550008 ,China) |
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| Abstract: | The bovine tuberculosis were to on the rise, the development of fast and efficient molecular biological detection method were needed. The specific primers of bacillus tuberculosis bovis were designed to develop the PCR assay and the reaction conditions were optimized. Results show that the PCR detection method for bacillus tuberculosis bovis were detected, the best template concentration, primer concentration and annealing temperature were 1.32μg/mL,0.5 μg/L, 54 ℃ ; Performance evaluation showed that the method has good specificity, sensitivity and clinical application. The develop- ment of the PCR assay provide efficient technology for prevention and control of bovine tuberculosis. |
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| Keywords: | Bacillus Tuberculosis Bovis PCR Detection Method Establish |
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