| 小麦育种亲本SW3243中1BL/1RS易位的细胞学和分子标记检测及来源分析 |
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| 引用本文： | 杨恩年,唐宗祥,李 俊,杨武云,邹裕春. 小麦育种亲本SW3243中1BL/1RS易位的细胞学和分子标记检测及来源分析[J]. 麦类作物学报, 2010, 30(5): 796-800 |
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| 作者姓名： | 杨恩年 唐宗祥 李 俊 杨武云 邹裕春 |
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| 作者单位： | 1. 四川省农科院作物所,四川,成都,610066
2. 四川农业大学植物遗传育种省级重点实验室,四川,雅安,625014 |
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| 基金项目： | 科技部国际合作项目四川CIMMYT合作选育条锈病微效多基因持久抗性小麦新品种（系）（2007DFA31480）。 |
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| 摘 要： | 为了深入了解小麦育种亲本SW3243的遗传基础,利用多重PCR引物对SW3243及其他47个材料进行1BL/1RS易位系检测,同时对SW3243进行Giemsa-C带分析,并利用5对定位于黑麦染色体1RS上的特异PCR引物对1BL/1RS易位系进行了多样性分析。多重PCR分析结果表明,包括SW3243在内共23个材料含有1BL/1RS易位。细胞学研究结果证实,SW3243含有1对1BL/1RS易位染色体;筛选到定位于黑麦1RS上的3对SSR引物(Xmwg913、Xmwg2062a、SCM9)可用于区别不同的1BL/1RS易位染色体;用筛选到的3对特异PCR引物对SW3243及其他22个1BL/1RS品种(系)进行分析,结果表明SW3243所含1BL/1RS易位染色体与SW22514、西科麦2号、山前麦、川麦32、川麦35的1 BL/1 RS易位染色体不同。
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| 关 键 词： | 小麦；SW3243；1BL/1RS；GiemsaC带；特异PCR |
Molecular and Cytogenetics Identification of Alien Chromation in Dwarf,Large Spike and High Yielding Potential Wheat Breeding Parent SW3243 |
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| YANG En nian,TANG Zong xiang,LI Jun,YANG Wu yun,ZOU Yu chun. Molecular and Cytogenetics Identification of Alien Chromation in Dwarf,Large Spike and High Yielding Potential Wheat Breeding Parent SW3243[J]. Journal of Triticeae Crops, 2010, 30(5): 796-800 |
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| Authors: | YANG En nian TANG Zong xiang LI Jun YANG Wu yun ZOU Yu chun |
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| Abstract: | The plant area of SW3243 and its derived varieties were totally extended to 4.33 million hectare in Sichuan, and 7 varieties derived from SW3243 were released in Sichuan province and 3 were released in national level. Giemsa C banding displayed that dwarf, large spike and high yielding wheat breeding parent SW3243 had a pair of chromosomes 1RS/ 1BL. Moreover, 3 Specific PCR primers were selected to test SW3243 and other 22 varieties, Specific PCR testing indicated that SW3243 contained 1RS/ 1BL, but its 1RS/ 1BL was different from these 1RS/ 1BL contained by varieties SW22514, Xikemai 2, Predgornia, Chuanmai 32 and Chuanmai 35. |
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