云南番茄褪绿病毒和番茄黄化曲叶病毒复合侵染的分子鉴定

在云南省元谋县发现大量下部叶片褪绿黄化，顶叶卷曲畸形的番茄植株。将叶片采集带回实验室，采用番茄褪绿病毒（Tomato chlorosis virus，ToCV）和番茄黄化曲叶病毒（Tomato yellow leaf curl virus，TYLCV）的特异性引物分别扩增得到774和400 bp的目标条带，测序比对发现其与ToCV的KR184676.1和TYLCV的FJ012359.1序列相似度均为99%，确认这两条带对应的两种病毒分别为ToCV和TYLCV，明确该番茄植株被ToCV和TYLCV复合侵染。同时发现云南地区有B型和Q型两种烟粉虱存在，且Q型的比例高于B型，二者均可携带ToCV和TYLCV。ToCV首次在云南省发现，其由沿海向内陆蔓延的过程中与TYLCV的复合侵染已蔓延至西南地区，值得高度重视和警惕。

Molecular Identification of Tomato chlorosis virus and Tomato yellow leaf curl virus in Yunnan Province

The tomato leaves that displayed symptoms of the chlorosis and yellowing on the lower leaves and symptoms of the curl and deformity on the upper leaves were found and collected in Yunnan. The target DNA band of Tomato chlorosis virus（ToCV）and Tomato yellow leaf curl virus（TYLCV），774 bp and 400 bp，respectively，was amplified using the special primer for virus. The target PCR products were sequenced and compared with the NCBI nucleotide database via the BLAST tools. The result showed that the sample had 99% similarity with the KR184676.1 of ToCV and the FJ012359.1 of TYLCV. We can confirm that the tomato was simultaneously infected by the ToCV and TYLCV. Meanwhile，we found that in Yunnan Province Bemisia tabaci B and Q coexisted，and the proportion of Q was higher than B. Both of B and Q could carry ToCV and TYLCV. This is the first report of ToCV in Yunnan Province. In the spread process from coast to interior areas，the co-infection of ToCV and TYLCV has transmitted to the Southwest of China，and we should pay high attention to the trend of the virus.