龙眼ELF4同源基因的克隆与功能研究

以‘红核子’龙眼（Dimocarpus longan）叶芽为材料，克隆了ELF4同源基因DlELF4-1和DlELF4-2的cDNA全长序列，对序列进行了生物信息学分析，并通过转化拟南芥以及实时荧光定量PCR研究基因功能。蛋白序列比对结果表明，DlELF4-2与AtELF4-L1有较高相似度，而DlELF4-1与AtELF4相似度更高；构建两个基因的植物表达载体转化拟南芥，转基因植株都表现出延迟开花以及不定根生长的现象，表明DlELF4-1和DlELF4-2可能有抑制开花和促进生长素合成功能；DlELF4-1和DlELF4-2在龙眼花芽分化过程的表达模式不同，推测其在龙眼花芽分化过程中有不同的功能。

Cloning and Function Analysis of ELF4 Homolog Genes in Dimocarpus longan

The full length of cDNA sequences of two ELF4 homolog genes were cloned from Dimocarpus longan‘Red Seed’，named DlELF4-1 and DlELF4-2. The functions of these two genes were analyzed by bioinformatics analysis and real-time quantitative PCR. To further study the function of the DlELF4 genes，DlELF4-1 and DlELF4-2 were overexpressed in Arabidopsis thaliana. Sequence analysis and cluster analysis showed that DlELF4-2 was similar to AtELF4-L1，while DlELF4-1 and AtELF4 showed higher similarity. Overexpression of two DlELF4 genes separately in Arabidopsis thaliana resulted in delaying flowering and adventitious roots phenotypes，suggesting that DlELF4 genes may be repressors of flowering and promoting auxin synthesis. However，expression patterns of DlELF4-1 and DlELF4-2 genes were different during bud differentiation of longan，indicating that the two genes may have different functions in longan.