| 波士顿蕨~(60)Co-γ射线辐射育种研究 |
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| 引用本文： | 余蓉培,王頔,李东,詹选怀,石雷.波士顿蕨~(60)Co-γ射线辐射育种研究[J].园艺学报,2018,45(5):988-996. |
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| 作者姓名： | 余蓉培 王頔 李东 詹选怀 石雷 |
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| 作者单位： | （1中国科学院植物研究所北方资源植物重点实验室，中国科学院植物研究所北京植物园，北京 100093；2云南农业科学院花卉研究所，国家观赏园艺工程技术研究中心，昆明 650205；3中国科学院庐山植物园，江西庐山 332900；4云南大学生命科学学院，昆明 650091） |
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| 摘 要： | 以波士顿蕨(Nephrolepis exaltata‘Bostoniensis Murano’)的绿色球状体(Green Globular Bodies,GGB)为辐照材料,采用不同剂量的~(60)Co-γ射线处理,结合组织培养技术进行育种研究。结果表明:绿色球状体辐照半致死剂量约为128 Gy。随着辐照剂量的增加,绿色球状体的增殖和分化受到抑制。绿色球状体辐射表型损伤表现为:颜色变深,变紧实,增殖和分化受抑制;细胞学损伤表现为:胚性细胞减少,出现微核。50~200 Gy剂量下,随着辐照剂量的增加,绿色球状体分化植株变矮,叶形变异率增加。在150 Gy剂量下筛选得到叶片变异性状显著的突变体Neg1。
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| 关 键 词： | 波士顿蕨 绿色球状体 60Co-γ射线 辐射育种 |
Radiation Breeding of Boston Fern via 60Co-γ Rays |
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| YU Rongpei,,WANG Di,LI Dong,ZHAN Xuanhuai,SHI Lei,.Radiation Breeding of Boston Fern via 60Co-γ Rays[J].Acta Horticulturae Sinica,2018,45(5):988-996. |
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| Authors: | YU Rongpei WANG Di LI Dong ZHAN Xuanhuai SHI Lei |
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| Institution: | （1The Key Laboratory of Plant Resources and Beijing Botanical Garden，Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China；2Flower Research Institute，Yunnan Academy of Agricultural Sciences，National Engineering Research Center for Ornamental Horticulture，Kunming 650205，China；3Lushan Botanical Garden，Chinese Academy of Sciences，Lushan, Jiangxi 332900，China；4School of Life Sciences，Yunnan University， Kunming 650091，China） |
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| Abstract: | Through the combination of tissue culture and radiation mutation，radiation breeding of Boston fern（Nephrolepis exaltata‘Bostoniensis Murano’）was investigated by using green globular bodies（GGB）as materials radiated by various dose of 60Co-γ rays. The results showed that the LD50 of GGB was about 128 Gy. With increasing dose of 60Co-γ rays，the multiplication and differentiation of Boston fern GGB were depressed. Morphology damage of Boston fern GGB radiated by 60Co-γ rays included darken color，increased density and the depression of multiplication and differentiation，and cellular damage included reduction of embryonic cell and initiation of cellular micronucleus. At 50–200 Gy，with increasing dose of 60Co-γ rays，the height of Boston fern plants differentiated from GGB became dwarf，and leaf mutation rate increased. Moreover，leaf mutant Neg1 was obtained at 150 Gy. |
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| Keywords: | Nephrolepis exaltata boston fern green globular bodies 60Co-γray radiation breeding |
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