| 乙烯响应因子ERF参与转基因菊花水培低氧胁迫耐受性的调控 |
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| 引用本文： | 刘晓芬,向理理,殷学仁,李方,陈昆松. 乙烯响应因子ERF参与转基因菊花水培低氧胁迫耐受性的调控[J]. 园艺学报, 2018, 45(1): 109-116. DOI: 10.16420/j.issn.0513-353x.2017-0184 |
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| 作者姓名： | 刘晓芬 向理理 殷学仁 李方 陈昆松 |
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| 作者单位： | 浙江省园艺作物整合重点实验室，浙江大学农业与生物技术学院，杭州 310058 |
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| 摘 要： | 将参与柿果实低氧胁迫响应的异源基因DkERF9转化到切花菊‘九月九’植株中，经PCR检测，获得了5株过量表达（35S︰︰DkERF9）的阳性植株。进一步比较分析了35S︰︰DkERF9植株与野生型植株的耐淹水性，发现水培低氧胁迫处理2周后，35S︰︰DkERF9植株较野生型植物生长缓慢，根系生长和茎的伸长均受到显著抑制，叶片颜色较淡；移除水培低氧胁迫处理3周后，生长部分恢复，但是叶片颜色及整体长势均弱于野生型植株。
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| 关 键 词： | 菊花 ERF 涝害 低氧胁迫 遗传转化 |
Ethylene Responsive Factors ERF Regulated the Hypoxia Response of Transformed Chrysanthemum Lines |
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| LIU Xiaofen,XIANG Lili,YIN Xueren,LI Fang,CHEN Kunsong. Ethylene Responsive Factors ERF Regulated the Hypoxia Response of Transformed Chrysanthemum Lines[J]. Acta Horticulturae Sinica, 2018, 45(1): 109-116. DOI: 10.16420/j.issn.0513-353x.2017-0184 |
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| Authors: | LIU Xiaofen XIANG Lili YIN Xueren LI Fang CHEN Kunsong |
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| Affiliation: | Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology，College of Agriculture & Biotechnology，Zhejiang University，Hangzhou 310058，China |
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| Abstract: | The exogenous DkERF9，a reported hypoxia responsive gene from persimmon，was transformed into‘Jiuyuejiu’chrysanthemum. Five positive lines（35S::DkERF9）were obtained and verified with PCR. Compared to the wild type plants，the growth of 35S::DkERF9 lines were inhibited significantly by water planting treatments for two weeks，as the length of roots and shoots were shorter in 35S::DkERF9 lines. The differences between 35S::DkERF9 lines and wild type plants remain significant，after three weeks recovery. |
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| Keywords: | chrysanthemum ERF waterlogging hypoxia transformation |
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