| 番茄组蛋白变体H2A.Z基因双突变体的获得及其功能研究 |
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| 引用本文： | 杨学东,田守波,朱为民,卢盼玲,王虹,王颖,张辉,朱龙英.番茄组蛋白变体H2A.Z基因双突变体的获得及其功能研究[J].园艺学报,2018,45(6):1081-1088. |
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| 作者姓名： | 杨学东 田守波 朱为民 卢盼玲 王虹 王颖 张辉 朱龙英 |
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| 作者单位： | 上海市农业科学院园艺研究所，上海市设施园艺技术重点实验室，上海 201403 |
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| 基金项目： | 国家重点研发计划项目（2016YFD0101703，2017YFD0101902）；上海市种业发展项目[沪农科种字（2016）第1-13号，沪农科种字（2017）第3-1-2号]；上海市农业科学院“卓越”团队建设计划项目[农科创2017（B-06）] |
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| 摘 要： | 以番茄自交系1479为材料，利用基因编辑技术创制番茄组蛋白变体H2A.Z基因双突变体hta11hta9。测序结果表明番茄H2A.Z基因被敲除。发芽试验结果表明组蛋白基因H2A.Z双突变体hta11hta9种子萌发受到严重影响。在相对低温（17 ℃）下，H2A.Z基因双突变体生长明显比野生型迟缓。
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| 关 键 词： | 番茄 基因编辑 组蛋白变体 |
Tomato Double Mutant of Histone Variant Gene was Obtained and its Function Research |
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| YANG Xuedong,TIAN Shoubo,ZHU Weimin,LU Panling,WANG Hong,WANG Ying,ZHANG Hui,ZHU Longying.Tomato Double Mutant of Histone Variant Gene was Obtained and its Function Research[J].Acta Horticulturae Sinica,2018,45(6):1081-1088. |
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| Authors: | YANG Xuedong TIAN Shoubo ZHU Weimin LU Panling WANG Hong WANG Ying ZHANG Hui ZHU Longying |
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| Institution: | The Protected Horticulture Institute，Shanghai Academy of Agricultural Sciences，Shanghai Key Laboratory of Protected Horticulture Technology，Shanghai 201403，China |
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| Abstract: | Using tomato inbred line 1 479 as the material，a double mutant of H2A.Z gene of histone variant hta11hta9 was obtained by gene editing technique. Sequencing analysis showed that the tomato H2A.Z gene was knocked out. The results of germination experiment showed that H2A.Z double mutant hta11hta9 had a serious effect on seed germination. The growth of H2A.Z double mutant hta11hta9 was obviously slower than wild type under the relative low temperature of 17 ℃. |
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| Keywords: | tomato gene editing histone variant |
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