| 家蚕卵线粒体DNA限制性内切酶长度多态性研究 |
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| 引用本文： | 廖顺尧,刘运强,鲁成,周泽扬,向仲怀. 家蚕卵线粒体DNA限制性内切酶长度多态性研究[J]. 西南大学学报(自然科学版), 2001, 23(1): 29-32 |
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| 作者姓名： | 廖顺尧 刘运强 鲁成 周泽扬 向仲怀 |
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| 作者单位： | 西南农业大学农业部蚕桑学重点开放实验室，重庆400716 |
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| 摘 要： | 利用29种限制性内切酶对34个不同品种家蚕卵mt DNA进行酶切分析，发现一化、二化品种与多化品种的HaeⅢ酶切图谱有差异，但不同的地理品种间没有出现酶切多态性现象。另外，一些家蚕品种的受精卵和非受精卵mt DNA在Bgl I和Pst I酶切焉，呈现不同的酶切带型。同时，绘制了较精确的家蚕mt DNA限制性内切酶酶切图谱。
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| 关 键 词： | 家蚕 线粒体DNA 限制性长度多态性 限制性内切酶 蚕卵 |
| 文章编号： | 1000-2642(2001)01-0029-04 |
| 修稿时间： | 2000-09-04 |
RESTRICTION ENDONUCLEASE FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF MITOCHONDRIA DNA IN EGGS OF BOMBYX MORI |
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| LIAO Shun-yao,LIU Yun-qiang,LU Cheng,ZHOU Ze-yang,XIANG Zhong-huai. RESTRICTION ENDONUCLEASE FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF MITOCHONDRIA DNA IN EGGS OF BOMBYX MORI[J]. Journal of southwest university (Natural science edition), 2001, 23(1): 29-32 |
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| Authors: | LIAO Shun-yao LIU Yun-qiang LU Cheng ZHOU Ze-yang XIANG Zhong-huai |
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| Abstract: | The restriction endonuclease maps of mitochondria DNA (mt DNA) from the eggs of 34 domestic silkworm ( Bombyx mori) strains were analyzed through 29 different restriction endonucleases.mtDNA of multivoltine strains showed different RFLP (restriction endonuclease length polymorphism) from that of monovoltine and bivoltine strains,but no RFLP was shown to exist between different geographical strains.RFLP was also observed between fertilized and unfertilized eggs of the same breed in their mtDNA.Restriction endonuclease maps with moderate preciseness were constructed of domestic silkworm. |
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| Keywords: | domestic silkworm ( Bombyx mori) mitochondria DNA(mtDNA) restriction fragment length polymorphism (RFLP) |
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